The Advances and Application of ABO Blood Group Genotyping Technology --Review.
10.19746/j.cnki.issn.1009-2137.2022.02.050
- Author:
Hui LI
1
;
Chen-Chen FENG
2
;
Qing CHEN
3
Author Information
1. Nanjing Medical University, Nanjing 211166, Jiangsu Province, China.
2. Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China.
3. Nanjing Medical University, Nanjing 211166, Jiangsu Province, China,Jiangsu Province Blood Center, Nanjing 210042, Jiangsu Province, China,E-mail:chenqing90@yahoo.com.
- Publication Type:Review
- Keywords:
ABO blood group;
blood group typing;
genotyping
- MeSH:
ABO Blood-Group System/genetics*;
Blood Grouping and Crossmatching;
Genotype;
Humans;
Polymerase Chain Reaction/methods*;
Technology
- From:
Journal of Experimental Hematology
2022;30(2):622-626
- CountryChina
- Language:Chinese
-
Abstract:
The ABO blood group system is the most important blood group system in clinical transfusion. Serological technology is a routine method for the identification of ABO blood groups, however, which have some limitations in the identification of complicated ABO samples with weakened antigens or antibodies, abnormal plasma proteins, polyagglutination, or cold agglutinin, etc. With the development of molecular biology technology, ABO blood group gene was cloned, and ABO blood group genotyping technology based on DNA was established. The genotyping technologies with different throughputs such as PCR-SSP, Droplet-AS-PCR, PCR-RFLP, PCR-SBT, SNaPshot, MALDI-TOF MS and NGS have emerged. Genotyping has overcome the limitations of serology, and has become an indispensable method to solve difficult blood type, providing strong support for the correct identification of ABO blood group, and providing guarantee for precision blood transfusion. This review summarizes the progress and application of ABO blood group genotyping methods.
