Expression and Clinical Significance of Serum MiR-424 and MiR-765 in Multiple Myeloma.
10.19746/j.cnki.issn.1009-2137.2022.02.023
- Author:
Juan-Juan HUANG
1
;
Fen ZHUO
2
;
Yue-Hong CAI
2
;
Hua XIAO
3
Author Information
1. Department of Hematology, The Third People's Hospital of Hainan Province, Sanya 572000, Hainan Province, China.
2. Department of Clinical Laboratory Examination, The Third People's Hospital of Hainan Province, Sanya 572000, Hainan Province, China.
3. Department of Clinical Laboratory Examination, The Third People's Hospital of Hainan Province, Sanya 572000, Hainan Province, China,E-mail: 537522863@qq.com.
- Publication Type:Journal Article
- Keywords:
clinical significance;
miR-424;
miR-765;
multiple myeloma
- MeSH:
Humans;
Immunoglobulin A;
Immunoglobulin G;
MicroRNAs;
Multiple Myeloma;
ROC Curve
- From:
Journal of Experimental Hematology
2022;30(2):461-465
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and clinical significance of miR-424 and miR-765 in patients with multiple myeloma (MM).
METHODS:The eighty-one MM patients admitted to Sanya Central Hospital from January 2017 to July 2020 were divided into phase Ⅰ (n=16), phase Ⅱ (n=25) and phase Ⅲ (n=40) according to the international staging system, while they were divided into IgG type (n=46), IgA type (n=19), light chain type (n=10) and non secretory type (n=6) according to the results of immunotyping. Another 50 healthy normal persons in the same period were selected as the control group. The levels of serum miR-424, miR-765 and Cystatin C (Cys-C) were measured in each group. The diagnostic value of serum miR-424, miR-765 and Cys-C in MM was estimated by ROC curve. Pearson correlation was used to analyze the correlation between serum levels of miR-424, miR-765 and Cys-C in MM patients.
RESULTS:The serum levels of miR-424 (2.74±1.30 vs 0.85±0.26), miR-765 (2.05±0.82 vs 0.63±0.17) and Cys-C ［(2.18±0.86 vs 0.72±0.15) mg/L］ in MM group were significantly higher than those in control group (P<0.001). The serum levels of miR-424 (5.08±2.36 vs 1.12±0.34, 2.24±0.93), miR-765 (3.50±1.52 vs 0.74±0.20, 1.78±0.65) and Cys-C ［(3.81±1.30 vs 0.92±0.24, 1.68±0.55) mg/L］ in MM patients at stage Ⅲ were significantly higher than those in patients at stage Ⅰ and Ⅱ (P<0.001). Also the serum levels of the three molecules in phase II were significantly higher than those in phase I (P<0.001). The serum levels of miR-424 and miR-765 in MM patients at IgG type were significantly higher than those at IgA, light chain and non secretory types (P<0.001). ROC curve analysis showed that the area under the curve (0.952，95%CI: 0.890-0.993) was greatest for the combination of miR-424, miR-765 and Cys-C for diagnosis of MM, and its sensitivity and specificity were 95.0% and 87.2%. The results of correlation analysis showed that the serum levels of miR-424 and miR-765 were positively correlated with Cys-C (r=0.795，r=0.760).
CONCLUSION:The serum levels of miR-424 and miR-765 in MM patients are significantly increased in the pattern increasing with the progression of MM stage. Combined with Cys-C, miR-424 and miR-765 have high value in the diagnosis of MM.
