Effect of Acute Myeloid Leukemia Cells on the Proliferation and Apoptosis of Bone Marrow-Derived Mesenchymal Stromal Cells.
10.19746/j.cnki.issn.1009-2137.2022.02.018
- Author:
Ze-Hui CHEN
1
,
2
,
3
;
Ya-Xin ZHENG
1
,
2
,
3
;
Jun-Qi SI
1
,
2
,
3
;
Tian YUAN
1
,
2
,
3
;
Zhi-Rong ZHANG
4
;
Chen TIAN
1
,
2
,
5
,
6
Author Information
1. Department of Hematology, Tianjin Medical University Cancer Institute and Hospital
2. National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin
3. Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.
4. Department of Oncology, Hetian District People's Hospital, Hetian 848000, Xinjiang Uygur Autonomous Region, China.
5. Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China
6. Department of Oncology, Hetian District People's Hospital, Hetian 848000, Xinjiang Uygur Autonomous Region, China,E-mail: tianchen@tjmuch.com.
- Publication Type:Journal Article
- Keywords:
acute myeloid leukemia;
apoptosis;
bone marrow-derived mesenchymal stromal cell;
mouse;
proliferation
- MeSH:
Animals;
Apoptosis;
Bone Marrow;
Bone Marrow Cells;
Cell Proliferation;
Humans;
Leukemia, Myeloid, Acute;
Mesenchymal Stem Cells;
Mice;
Tumor Microenvironment
- From:
Journal of Experimental Hematology
2022;30(2):430-434
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of acute myeloid leukemia cells in leukemia-microenvironment on proliferation and apoptosis of bone marrow-derived mesenchymal stromal cells (BM-MSC).
METHODS:Acute myeloid leukemia (AML) murine models overexpressing MLL-AF9 were established. The number of BM-MSC of wild type (WT) and AML-derived mice were analyzed by flow cytometry. Morphology and growth differences between WT and AML-derived BM-MSC were analyzed by inverted fluorescence microscope. Proliferation and apoptosis of BM-MSC between these two groups were detected by Brdu and Annexin V/PI.
RESULTS:Compared with WT-derived BM-MSC, the number and proliferation rate of AML-derived BM-MSC significantly increased (P<0.01, P<0.001), while apoptosis rate decreased (P<0.05). When cultured in vitro, BM-MSC grew faster under conditional medium.
CONCLUSION:AML cells can promote proliferation and inhibit apoptosis of BM-MSC.
