Effect of Carvacrol on the Biological Behavior of Leukemia Cells and Its Mechanism.
10.19746/j.cnki.issn.1009-2137.2022.02.012
- Author:
Yan LIANG
1
;
Ai-Ying YANG
1
;
Min LIU
1
;
Yan-Juan CHENG
1
;
Si-Bin ZANG
1
;
Jun HUANG
2
;
Yuan-Yan TANG
1
;
Zhi-Ping HUANG
3
Author Information
1. Department of Hematology, Jingzhou Central Hospital, Jingzhou 434020, Hubei Province, China.
2. Laboratory of Hematology, Jingzhou Central Hospital, Jingzhou 434020, Hubei Province, China.
3. Department of Hematology, Jingzhou Central Hospital, Jingzhou 434020, Hubei Province, China,E-mail：191060635@qq.com.
- Publication Type:Journal Article
- Keywords:
carvacrol;
leukemia;
cell apoptosis;
cell proliferation;
circ-0008717;
miR-217
- MeSH:
Antagomirs;
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Cymenes;
Humans;
Leukemia;
MicroRNAs/genetics*
- From:
Journal of Experimental Hematology
2022;30(2):393-399
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of carvacrol on the biological behavior of leukemia cells and its regulation to circ-0008717/miR-217 molecular axis.
METHODS:Human acute lymphoblastic leukemia cells Molt-4 were cultured in vitro, and different concentrations of carvacrol were added to the cells. si-NC and si-circ-0008717 were transfected into Molt-4 cells (si-NC group, si-circ-0008717 group). pcDNA, pcDNA-circ-0008717, anti-miR-NC, anti-miR-217 were transfected into Molt-4 cells and then added to carvacrol-treated cells (carvacrol+pcDNA group, carvacrol+pcDNA-circ-0008717 group, carvacrol+anti-miR-NC group, carvacrol+anti-miR-217 group). MTT, plate clone formation experiment, and flow cytometry were used to detect the viability of the cell, colony formation number, and apoptosis rate of cells, respectively. The RT-qPCR method was used to detect the expression levels of circ-0008717 and miR-217. The dual luciferase reporter gene experiment was used to detect the targeting relationship between circ-0008717 and miR-217.
RESULTS:After carvacrol treatment, the cell viability decreased significantly (r=-0.9405), expression level of circ-0008717 decreased (r=-0.9117), colonies formed number decreased (r=-0.9256), while the cell apoptosis rate increased (r= 0.8464), and the expression level of miR-217 increased (r=0.9468). Compared with the si-NC group, the expression level of miR-217 in si-circ-0008717 group increased (P＜0.001), the cell apoptosis rate increased (P＜0.001), while cell viability decreased (P＜0001), the number of colonies formed decreased (P＜0.001). Compared with the carvacrol+pcDNA group, the cell viability of the carvacrol+pcDNA-circ-0008717 group increased (P＜0.001), the number of colonies formed increased (P＜0.001), while the cell apoptosis rate decreased (P＜0.001). circ-0008717 could target miR-217. The cell viability of the carvacrol+anti-miR-217 group increased (P＜0.001), and the number of colonies formed increased (P＜0.001), while the cell apoptosis rate decreased (P＜0001) as compared with the carvacrol+anti-miR-NC group.
CONCLUSION:Carvacrol can promote the expression of miR-217 by down-regulating the expression of circ-0008717, thereby reducing the proliferation and cloning ability of leukemia cells and promoting cell apoptosis.
