Effect of Rheb1 in the Development of Mouse Megakaryocyte-Erythroid Progenitor Cells.
10.19746/j.cnki.issn.1009-2137.2022.01.049
- Author:
Juan GAO
1
;
Shuang YANG
2
;
Yu-Xia WANG
2
;
Ya-Nan GAO
3
;
Ya-Jing CHU
2
;
Wei-Ping YUAN
2
;
Xiao-Min WANG
2
Author Information
1. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Hematological Disorders, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China,Tianjin Eye Hospital, Tianjin Key Laboratory of Ophthalmology and Visual Science, Tianjin Eye Institute, Clinical College of Ophthalmology, Tianjin Medical University, Nankai University Affiliated Eye Hospital, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Hematological Disorders, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
3. Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin Medical University, Tianjin 300060, China.
- Publication Type:Journal Article
- Keywords:
Rheb1;
Rheb1;
Vav1-Cre;
megakaryocyte-erythroid progenitor cells;
mouse;
rapamycin
- MeSH:
Animals;
Cell Differentiation;
Erythrocytes;
Flow Cytometry;
Megakaryocyte-Erythroid Progenitor Cells;
Megakaryocytes;
Mice;
Signal Transduction
- From:
Journal of Experimental Hematology
2022;30(1):298-304
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of Rheb1 in the development of mouse megakaryocyte-erythroid progenitor cells and its related mechanism.
METHODS:Rheb1 was specifically knocked-out in the hematopoietic system of Vav1-Cre;Rheb1fl/fl mice(Rheb1Δ/Δ mice). Flow cytometry was used to detect the percentage of red blood cells in peripheral blood and erythroid cells in bone marrow in Vav1-Cre;Rheb1fl/fl mice and control mice. The CFC assay was used to detect the differentiation ability of Rheb1 KO megakaryocyte-erythroid progenitor cells and control cells. Real-time fluorescence quantification PCR was used to detect the relative expression of PU.1,GATA-1,GATA-2,CEBPα and CEBPβ of Rheb1 KO megakaryocyte-erythroid progenitor cells and control cells. Rapamycin was added to the culture medium, and it was used to detect the changes in cloning ability of megakaryocyte-erythroid progenitor cells from wild-type mice in vitro.
RESULTS:After Rheb1 was knocked out, the development and stress response ability of megakaryocyte-erythroid progenitor cells in mice were weaken and the differentiation ability of megakaryocyte-erythroid progenitor cells in vitro was weaken. Moreover, the expression of GATA-1 of megakaryocyte-erythroid progenitor cells was decreased. Further, rapamycin could inhibit the differentiative capacity of megakaryocyte-erythroid progenitor cells in vitro.
CONCLUSION:Rheb1 can regulate the development of megakaryocyte-erythroid progenitor cells probably through the mTOR signaling pathway in mice.
