The Establishment and Application of Flow Cytometry on the Detection of Jka Antigen in Kidd Blood Group.
10.19746/j.cnki.issn.1009-2137.2022.01.042
- Author:
Hao ZHANG
1
;
Shuang LIANG
1
;
Le-Bing LI
2
;
Yu-Qing SU
3
Author Information
1. Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
2. School of Life and Marine Sciences, Shenzhen University, Shenzhen 518061, Guangdong Province, China.
3. Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China,E-mail: 1435133589@qq.com.
- Publication Type:Journal Article
- Keywords:
Kidd blood group;
JKA gene;
antigen Jka;
flow cytometry
- MeSH:
Blood Group Antigens;
Blood Grouping and Crossmatching;
Erythrocytes;
Flow Cytometry;
Humans;
Immunoglobulin G;
Kidd Blood-Group System
- From:
Journal of Experimental Hematology
2022;30(1):250-255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a based method flow cytometry to identify the antigen Jka in human red blood cells (RBCs) and verify its accuracy.
METHODS:A total of 96 blood samples were enrolled in the study randomly from the voluntary blood donors in Shenzhen Blood Center. The RBCs were incubated with IgG anti-Jka primary antibody, and then labeled with the secondary antibody anti-IgG-Alexa Fluor 647. The fluorescence histograms of each sample were obtained by flow cytometry. Serological agglutination test was used to compare the accuracy of flow cytometry in the detecting of antigen Jka, while PCR-SSP and gene sequencing genotyping were used to verify the accuracy of flow cytometry in the detecting of the antigen in human RBCs.
RESULTS:The results of flow cytometry for antigen Jka in human RBCs were consistent with those from serological tests. Samples that demonstrated higher serological agglutination intensity also showed higher fluorescence activity, which indicate more stronger of Jka antigen. The sensitivity of flow cytometry was higher than that of serological test; especially in distinguish Jka weak and negative samples. Flow cytometric results of all samples were consistent with the genotyping results, which confirmed the accuracy of flow cytometry.
CONCLUSION:The study established a new flow cytometry-based method successfully for the identification of Jka antigen of Kidd blood group in human RBCs. The Kidd blood group antigen Jka of different intensities can be accurately distinguished by the technique.
