The Relationship between MicroRNA Expression Profiling in Imatinib-Resistant Cell Line K562/G and Potential Mechanism through FOXO3/Bcl-6 Signaling Pathway.
10.19746/j.cnki.issn.1009-2137.2022.01.017
- Author:
Jing SHEN
1
;
Huan WANG
1
;
Jing-Shi WANG
1
;
Na WEI
1
;
Zhao WANG
2
Author Information
1. Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China.
2. Department of Hematology, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing 100050, China,E-mail: wangzhao@ccmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Bcl-6;
FOXO3;
chronic myeloid leukemia;
drug resistance;
miRNA
- MeSH:
Forkhead Box Protein O3/genetics*;
Humans;
Imatinib Mesylate/pharmacology*;
K562 Cells;
MicroRNAs/genetics*;
RNA, Messenger;
Signal Transduction
- From:
Journal of Experimental Hematology
2022;30(1):107-112
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the drug resistant related FOXO3/Bcl-6 signaling pathway in K562/G cell line and its related microRNA(miRNA) mechanisms.
METHODS:The drug resistance potency of imatinib on K562/G was detected by MTT assay. The expression of FOXO3 and Bcl-6 proteins in K562 and K562/G cells was detected by Western blot. Real-time PCR (RT-PCR) was used to detect the expression of FOXO3 and Bcl-6 mRNA. The miRNA expression profiling in K562 and K562/G cells was analyzed by microarray technique, and the miRNA targeted to FOXO/Bcl-6 signaling pathway was identified.
RESULTS:The expression of FOXO3 and Bcl-6 protein was significantly increased in K562/G cells as compared with that in K562 cells (P<0.01), the expression level of Bcl-6 mRNA showed no increase in K562/G cells. However, FOXO3 mRNA was up-regulated in K562/G cells (P<0.05). MiRNA microarray results showed that 109 miRNAs were expressed differentially in K562 and K562/G cells. The expression of 81 miRNAs were up-regulated while 28 miRNAs were down-regulated. Through reverse prediction by bioinformatics, miR-6718-5p, miR-5195-5p, miR-4711-3p, miR-4763-5p, miR-4664-5p and miR-3176 were related to FOXO/Bcl-6 signaling pathway.
CONCLUSION:The FOXO3/Bcl-6 signaling pathway contributes to imatinib resistance in K562/G cell line, and the miRNA expression profiles showed significant differences between K562/G and K562 cells.
