Effects of Monoammonium Glycyrrhizinate on Stem Cell-like Characteristics, Oxidative Stress and Mitochondrial Function of Acute Promyelocytic Leukemia NB4 Cells.
10.19746/j.cnki.issn.1009-2137.2022.01.005
- Author:
Yan CHEN
1
;
Xian-Xiu WEN
2
,
3
;
Chen-Yan ZHOU
4
;
Rong ZHANG
4
;
Bing-Ju WU
4
;
Wei YU
4
Author Information
1. School of Nursing, Southwest Medical University, Luzhou 646000, Sichuan Province, China,Department of Pediatric, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610072, Sichuan Province, China.
2. School of Nursing, Southwest Medical University, Luzhou 646000, Sichuan Province, China
3. Nursing Department, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610072, Sichuan Province, China,E-mail:wxxjyc@163.com.
4. Department of Pediatric, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610072, Sichuan Province, China.
- Publication Type:Journal Article
- Keywords:
CD133;
NB4;
acute promyelocytic leukemia;
mitochondria;
monoammonium glycyrrhizinate;
oxidative stress
- MeSH:
Apoptosis;
Cell Line, Tumor;
Doublecortin-Like Kinases;
Humans;
Intracellular Signaling Peptides and Proteins/metabolism*;
Leukemia, Promyelocytic, Acute;
Mitochondria;
Oxidative Stress;
Protein Serine-Threonine Kinases;
Stem Cells
- From:
Journal of Experimental Hematology
2022;30(1):22-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of monoammonium glycyrrhizinate on the stem cell-like characteristics, oxidative stress and mitochondrial function of acute promyelocytic leukemia cells NB4.
METHODS:CCK-8 method was used to detect the viability of acute promyelocytic leukemia cells NB4, and the appropriate dose was screened; Cloning method was used to detect the proliferation rate of NB4 cell; Western blot was used to detect the expression of cell cycle-related protein; flow cytometry was used to detect cell apoptosis and sort NB4 stem cells positive (CD133+); Stem cell markers (Oct4, ABCG2, Dclk1) were detected by RT-PCR; ROS was detected by fluorescence; The kit was used to detect the level of oxidative stress markers (MDA); The flow cytometry was used to detect the change of mitochondrial membrane potential; Western blot was used to detect the expression of mitochondrial damage index-related proteins (Bax/BCL-2).
RESULTS:Compared with the control group, if the concentration of MAG was less than 5 μmol/L, the cell NB4 viability showed no significant difference; if the concentration was higher than 5 μmol/L, the inhibitory effect on the growth of cell NB4 increased and showed significant difference (P<0.05), according to the results of CCK-8 experiment, four groups were set based on the concentration of MAG 0 μmol/L, MAG 5 μmol/L, MAG 10 μmol/L, and MAG 20 μmol/L; compared with the control group (MAG 0 μmol/L), the cells in MAG 5 μmol/L group showed no significant difference, while the proliferation rate, cyclin expression, mitochondrial membrane potential, stem cell CD133+ ratio, and marker mRNA level ( Oct4, ABCG2, Dclk1) of NB4 cell were significantly reduced (P<0.05); the apoptosis rate, reactive oxygen species, MDA content and Bax/BCL-2 expression of NB4 cell significantly increased (P<0.05).
CONCLUSION:Monoammonium glycyrrhizinate has a significant inhibitory effect on acute promyelocytic leukemia cells NB4, which may be related to the regulation of stem cell-like characteristics, oxidative stress and mitochondrial function.
