Clinical value of metagenomic next-generation sequencing in complicated infectious diseases.
10.7499/j.issn.1008-8830.2110064
- Author:
Si-Yu HAN
1
Author Information
1. Department of Respiratory Medicine, Hebei Children's Hospital Affiliated to Hebei Medical University, Shijiazhuang 050031, China.
- Publication Type:Review
- Keywords:
Child;
Infectious disease;
Metagenomic next-generation sequencing;
Pathogen detection
- MeSH:
Bacteria;
Communicable Diseases/diagnosis*;
High-Throughput Nucleotide Sequencing/methods*;
Humans;
Metagenomics/methods*;
Sensitivity and Specificity
- From:
Chinese Journal of Contemporary Pediatrics
2022;24(2):210-215
- CountryChina
- Language:English
-
Abstract:
Infectious diseases are commonly seen in clinical practice, and pathogen diagnosis is the key link in diagnosis and treatment; however, conventional pathogen detection methods cannot meet clinical needs due to time-consuming operation and low positive rate. As a new pathogen detection method, metagenomic next-generation sequencing (mNGS) has a wide detection range and can detect bacteria, viruses, fungi, parasites, rare pathogens, and even unknown pathogens. The technique of mNGS is unbiased and can rapidly, efficiently, and accurately obtain all nucleic acid information in test samples, analyze pathogens, and guide clinical diagnosis and treatment, thereby playing an important role in complicated infectious diseases. This article reviews the diagnostic advantages and clinical value of mNGS in bacterial, fungal, viral, and parasitic infections.
