Molecular analysis of 23 cases of B subgroup.
10.3760/cma.j.cn511374-20210114-00041
- Author:
Jinhui XIE
1
;
Shuangyu LI
;
Mengli XUE
;
Lina WU
;
Ying ZHAO
;
Xian HUANG
;
Jinghui CHONG
;
Wei WANG
;
Zheng DONG
;
Bo SUN
;
Tongtong LI
;
Shiping AN
;
Lixin LI
Author Information
1. Tianjin Blood Center, Tianjin 300110, China. xie_jinhui@163.com.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System/genetics*;
Alleles;
Exons;
Genotype;
Humans;
Nucleotides;
Phenotype
- From:
Chinese Journal of Medical Genetics
2022;39(5):546-547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the molecular reasons of weak expression of B antigen on the red cell.
METHODS:Serological test for blood group was carried out, including red cell and plasma grouping, and anti-A1 and anti-H testing, and confirming weak A or B antigens by adsorption and elution. Exons 1-7 were sequenced directly, and one of them was cloned and sequenced.
RESULTS:All of the 23 samples showed the weak B antigen by serological method. The alleles of the subgroups were identified by DNA sequencing, including 2 Bel subgroup, 4 B3 subgroup, 14 Bw subgroup, 2 CisAB subgroup and a novel allele. The novel allele showed a nucleotide substitution 662G>A in the exon 7, and the sequence was submitted to Blood Group Antigen Gene Mutation Database, and the novel allele was named Bel10.
CONCLUSION:Nucleotide substitution in exon results in blood subgroup, which showed that the antigens were weakened, and Bw phenotype was the most frequently subgroup.
