Effect of BDNF-AS/miR-145-5p axis on renal tubular epithelial cell injury induced by high glucose.
10.3760/cma.j.cn511374-20201202-00841
- Author:
Qianqian JIA
1
;
Donghai WANG
Author Information
1. Department of Nephrology, Zhumadian Central Hospital, Zhumadian, Henan 463000, China. heart07@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Brain-Derived Neurotrophic Factor/genetics*;
Cell Proliferation;
Epithelial Cells;
Glucose;
Humans;
MicroRNAs/genetics*
- From:
Chinese Journal of Medical Genetics
2022;39(2):157-160
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect and possible mechanism of BDNF-AS on renal tubular epithelial cell injury induced by high glucose.
METHODS:Human renal tubular epithelial cells HK-2 were cultured in vitro and transfected with BDNF-AS small interfering RNA or miR-145-5p mimic, or co-transfected with BDNF-AS small interfering RNA and miR-145-5p inhibitor, respectively. The cells were then intervened with 30 mmol/L glucose for 24 hours. The expression of BDNF-AS and miR-145-5p were detected by RT-qPCR. Cell proliferation was detected by CCK-8, and apoptosis was detected by flow cytometry. The expression of Bcl-2 and Bax proteins were detected by Western blotting, and the levels of IL-1β and IL-6 in cell culture supernatant were detected by enzyme-linked immunosorbent assay. Dual luciferase reporter gene experiment was used to verify the regulatory relationship of BDNF-AS with miR-145-5p.
RESULTS:High glucose promoted the expression of BDNF-AS in HK-2 cells (P<0.05), but inhibited that of miR-145-5p (P<0.05). Interfering with BDNF-AS or overexpression of miR-145-5p decreased the inhibition rate, apoptosis rate and expression of Bax protein, IL-1β and IL-6 of HK-2 cells induced by high glucose (P<0.05), but promoted the expression of Bcl-2 protein (P<0.05). Interfering with miR-145-5p reversed the effect of interfering with BDNF-AS on the proliferation, apoptosis rate and the expression of IL-1β and IL-6 of HK-2 cells induced by high glucose. BDNF-AS could target and down-regulate miR-145-5p.
CONCLUSION:Interfering with BDNF-AS may promote the proliferation of renal tubular epithelial cells induced by high glucose and inhibit cell apoptosis and the expression of inflammatory factor by down-regulating miR-145-5p.
