Design, simulation and application of multichannel microfluidic chip for cell migration.
10.7507/1001-5515.202105002
- Author:
Huilai LI
1
;
Xiao YANG
2
;
Xiaosong WU
1
;
Zhigang LI
1
;
Chenggang HONG
3
;
Yong LIU
1
;
Ling ZHU
1
;
Ke YANG
1
Author Information
1. Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, P. R. China.
2. School of Biomedical Engineering, Anhui Medical University, Hefei 230032, P. R. China.
3. Hefei Zhongke Yikangda Biomedical Co., Ltd., Hefei 230031, P. R. China.
- Publication Type:Journal Article
- Keywords:
Advanced glycation end products;
Chemotaxis;
Inhibition;
Microfluidic concentration gradient chip;
Neutrophils
- MeSH:
Cell Movement;
Chemotaxis;
Equipment Design;
Lab-On-A-Chip Devices;
Microfluidic Analytical Techniques;
Microfluidics;
Neutrophils;
Reproducibility of Results
- From:
Journal of Biomedical Engineering
2022;39(1):128-138
- CountryChina
- Language:Chinese
-
Abstract:
Cell migration is defined as the directional movement of cells toward a specific chemical concentration gradient, which plays a crucial role in embryo development, wound healing and tumor metastasis. However, current research methods showed low flux and are only suitable for single-factor assessment, and it was difficult to comprehensively consider the effects of other parameters such as different concentration gradients on cell migration behavior. In this paper, a four-channel microfluidic chip was designed. Its characteristics were as follows: it relied on laminar flow and diffusion mechanisms to establish and maintain a concentration gradient; it was suitable for observation of cell migration in different concentration gradient environment under a single microscope field; four cell isolation zones (20 μm width) were integrated into the microfluidic device to calibrate the initial cell position, which ensured the accuracy of the experimental results. In particular, we used COMSOL Multiphysics software to simulate the structure of the chip, which demonstrated the necessity of designing S-shaped microchannel and horizontal pressure balance channel to maintain concentration gradient. Finally, neutrophils were incubated with advanced glycation end products (AGEs, 0, 0.2, 0.5, 1.0 μmol·L -1), which were closely related to diabetes mellitus and its complications. The migration behavior of incubated neutrophils was studied in the 100 nmol·L -1 of chemokine (N-formylmethionyl-leucyl-phenyl-alanine) concentration gradient. The results prove the reliability and practicability of the microfluidic chip.
