Study on the effect of small alcohol on lipid hydration and liposome formation.
10.7507/1001-5515.202105060
- Author:
Lihua JIANG
1
;
Qiong WANG
2
;
Ning HU
3
;
Jun YANG
3
Author Information
1. School of General Education, Chongqing City Management College, Chongqing 400055, P. R. China.
2. Department of Basic Medicine, Chongqing Medical and Pharmaceutical College, Chongqing 401331, P. R. China.
3. Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, P. R. China.
- Publication Type:Journal Article
- Keywords:
Biomembrane;
Ethanol;
Hydration;
Liposome
- MeSH:
Ethanol/pharmacology*;
Lipids;
Liposomes
- From:
Journal of Biomedical Engineering
2022;39(1):112-119
- CountryChina
- Language:Chinese
-
Abstract:
Liposomes with precisely controlled composition are usually used as membrane model systems to investigate the fundamental interactions of membrane components under well-defined conditions. Hydration method is the most common method for liposome formation which is found to be influenced by composition of the medium. In this paper, the effects of small alcohol (ethanol) on the hydration of lipid molecules and the formation of liposomes were investigated, as well as its coexistence with sodium chloride. It was found that ethanol showed the opposite effect to that of sodium chloride on the hydration of lipid molecules and the formation of liposomes. The presence of ethanol promoted the formation of liposomes within a certain range of ethanol content, but that of sodium chloride suppressed the liposome formation. By investigating the fluorescence intensity and continuity of the swelled membranes as a function of contents of ethanol and sodium chloride, it was found that sodium chloride and ethanol showed the additive effect on the hydration of lipid molecules when they coexisted in the medium. The results may provide some reference for the efficient preparation of liposomes.
