Effects of transcranial magneto-acoustic electrical stimulation on calcium signals in prefrontal nerve clusters.
10.7507/1001-5515.202107044
- Author:
Shuai ZHANG
1
;
Jiankang WU
1
;
Jiayue XU
1
;
Junwu DANG
1
;
Yihang ZHAO
1
;
Wentao HOU
1
;
Guizhi XU
1
Author Information
1. State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, P. R. China.
- Publication Type:Journal Article
- Keywords:
Calcium concentration;
Neuromodulation;
Optical fiber photometric detection technology;
Transcranial magneto-acoustic-electrical stimulation;
Ultrasonic stimulation
- MeSH:
Acoustics;
Animals;
Brain;
Calcium;
Electric Stimulation;
Mice;
Prefrontal Cortex;
Transcranial Direct Current Stimulation;
Transcranial Magnetic Stimulation
- From:
Journal of Biomedical Engineering
2022;39(1):19-27
- CountryChina
- Language:Chinese
-
Abstract:
Transcranial magneto-acoustic electrical stimulation (TMAES) is a novel method of brain nerve regulation and research, which uses induction current generated by the coupling of ultrasound and magnetic field to regulate neural electrical activity in different brain regions. As the second special envoy of nerve signal, calcium plays a key role in nerve signal transmission. In order to investigate the effect of TMAES on prefrontal cortex electrical activity, 15 mice were divided into control group, ultrasound stimulation (TUS) group and TMAES group. The TMAES group received 2.6 W/cm 2 and 0.3 T of magnetic induction intensity, the TUS group received only ultrasound stimulation, and the control group received no ultrasound and magnetic field for one week. The calcium ion concentration in the prefrontal cortex of mice was recorded in real time by optical fiber photometric detection technology. The new object recognition experiment was conducted to compare the behavioral differences and the time-frequency distribution of calcium signal in each group. The results showed that the mean value of calcium transient signal in the TMAES group was (4.84 ± 0.11)% within 10 s after the stimulation, which was higher than that in the TUS group (4.40 ± 0.10)% and the control group (4.22 ± 0.08)%, and the waveform of calcium transient signal was slower, suggesting that calcium metabolism was faster. The main energy band of the TMAES group was 0-20 Hz, that of the TUS group was 0-12 Hz and that of the control group was 0-8 Hz. The cognitive index was 0.71 in the TMAES group, 0.63 in the TUS group, and 0.58 in the control group, indicating that both ultrasonic and magneto-acoustic stimulation could improve the cognitive ability of mice, but the effect of the TMAES group was better than that of the TUS group. These results suggest that TMAES can change the calcium homeostasis of prefrontal cortex nerve clusters, regulate the discharge activity of prefrontal nerve clusters, and promote cognitive function. The results of this study provide data support and reference for further exploration of the deep neural mechanism of TMAES.
