Effects of Angong Niuhuang Pills against heart failure based on cross-scale polypharmacological study.
10.19540/j.cnki.cjcmm.20211206.401
- Author:
Xue-Jing SHI
1
;
Qiang JIN
2
;
Yu ZHAO
1
;
Mai-Cheng XU
2
;
Han ZHANG
3
;
Hong-da SHENG
3
;
Yi WANG
3
;
Xiao-Ping ZHAO
4
Author Information
1. School of Basic Medical Sciences, Zhejiang Chinese Medical University Hangzhou 310053, China.
2. Hangzhou Hu Qing Yu Tang Pharmaceutical Co., Ltd. Hangzhou 311100, China.
3. College of Pharmaceutical Sciences, Zhejiang University Hangzhou 310058, China.
4. School of Basic Medical Sciences, Zhejiang Chinese Medical University Hangzhou 310053, China Academy of Chinese Medical Science, Zhejiang Chinese Medical University Hangzhou 310053, China.
- Publication Type:Journal Article
- Keywords:
Angong Niuhuang Pills;
cross-scale fusion analysis;
differential metabolites;
heart failure;
pathway analysis;
polypharmacology
- MeSH:
Animals;
Biomarkers;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Heart Failure/drug therapy*;
Metabolomics;
Mice
- From:
China Journal of Chinese Materia Medica
2022;47(7):1888-1896
- CountryChina
- Language:Chinese
-
Abstract:
Angong Niuhuang Pills(AGNHP) are effective in clearing heat, removing the toxin, and eliminating phlegm for resuscitation. Clinically, it is widely used to treat various diseases such as febrile convulsion due to heat attacking pericardium, but its therapeutic effects on heart failure(HF) have not been well recognized. In this study, the profiles of differential metabolites regulated by AGNHP were identified by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS). The underlying mechanism of AGNHP against HF was illustrated based on the integrated analysis of pharmacological data and metabolic molecular network. The HF model was induced by isoproterenol in mice. After oral administration of AGNHP for one week, cardiac functions in HF mice were evaluated by echocardiography, and serum samples of mice were collected for metabolomics analysis. Eight differential metabolites of AGNHP against HF were screened out through partial least square discriminant analysis(PLS-DA) and input into MetaboAnalyst for the analysis of metabolic pathways. Moreover, the critical metabolic pathways regulated by AGNHP were enriched according to the potential targets of major compounds in AGNHP. After AGNHP treatment, the recovered index of relative content of some metabolites underwent cross-scale fusion analysis with therapeutic efficacy data, followed by "compound-reaction-enzyme-gene" network analysis. It is inferred that the anti-HF effects of AGNHP may be attributed to the metabolism of arachidonic acid, amino acid, glycerophospholipid, and linoleic acid. The cross-scale polypharmacological analysis method developed in this study provides a new method to interpret scientific principles of AGNHP against HF with modern technologies.
