Exploration of nanofiltration conditions for separation of alcohol precipitation liquid during preparation of Reduning Injection by Box-Behnken design combined with flux attenuation.
10.19540/j.cnki.cjcmm.20211122.301
- Author:
Cun-Yu LI
1
;
Jia-Li JIANG
2
;
Yong-Tuo ZHANG
2
;
Yu-Cen ZOU
2
;
Xing-Lei ZHI
2
;
Guo-Ping PENG
1
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Jiangsu Kanion Parmaceutical Co., Ltd. Lianyungang 222001, China State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process Lianyungang 222001, China Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Nanjing 210023, China Jiangsu Province Engineering Research Center of Classical Prescription Nanjing 210023, China.
2. College of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
3,5-dicaffeoylquinic acid;
4,5-dicaffeoylquinic acid;
Reduning Injection;
chlorogenic acid;
nanofiltration
- MeSH:
Chlorogenic Acid;
Drugs, Chinese Herbal/chemistry*;
Ethanol;
Injections
- From:
China Journal of Chinese Materia Medica
2022;47(7):1881-1887
- CountryChina
- Language:Chinese
-
Abstract:
This study employed Box-Behnken design combined with flux attenuation to explore the nanofiltration conditions for separation of alcohol precipitation liquid during the preparation of Reduning Injection and discussed the applicability of nanofiltration in the separation of the liquid with high-concentration ethanol. The effects of nanofiltration molecular weight cut-off(MWCO) and pH on the rejection of chlorogenic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid were consistent with the principles of pore size sieving and charge effect, respectively. The rejection of the three phenolic acids was reduced by concentration polarization effect caused by trans-membrane pressure(TMP). The swelling of membrane surface decreased the pore size and membrane flux for effective separation. Chlorogenic acid and 4,5-dicaffeoylquinic acid were more sensitive to pH and ethanol concentration than 3,5-dicaffeoylquinic acid. A certain correlation existed between the compound structure and the separation factors of nanofiltration, and the separation rules were associated with the comprehensive effect of charge effect, pore size sieving, concentration polarization, steric hindrance and so on.
