Integrated smart hyperspectral imaging and CARS-based characteristic band selection for rapid determination of SO_2 content in sulphur-fumigated Achyranthis Bidentatae Radix.
10.19540/j.cnki.cjcmm.20210707.302
- Author:
En-Ci JIANG
1
;
Lin CHEN
1
;
Ji-Zhong YAN
1
;
Yi TAO
1
Author Information
1. College of Pharmaceutical Science, Zhejiang University of Technology Hangzhou 310014, China.
- Publication Type:Journal Article
- Keywords:
Achyranthis Bidentatae Radix;
SO_2;
competitive adaptive reweighted sampling(CARS);
hyperspectral imaging;
quantitative model
- MeSH:
Hyperspectral Imaging;
Least-Squares Analysis;
Plant Roots;
Sulfur
- From:
China Journal of Chinese Materia Medica
2022;47(7):1864-1870
- CountryChina
- Language:Chinese
-
Abstract:
In order to realize the rapid and non-destructive detection of SO_2 content in sulphur-fumigated Achyranthis Bidentatae Radix, this paper first prepared the sulphur-fumigated Achyranthis Bidentatae Radix samples with the usage amount of sulphur being 0, 2.5%, and 5% of the mass of Achyranthis Bidentatae Radix pieces. The SO_2 content in different batches of sulphur-fumigated Achyranthis Bidentatae Radix was determined using the method in Chinese Pharmacopoeia, followed by the acquisition of their hyperspectral data within both visible-near infrared(435-1 042 nm) and short-wave infrared(898-1 751 nm) regions by hyperspectral imaging. Meanwhile, the first derivative, AUTO, multiplicative scatter correction, Savitzky-Golay(SG) smoothing, and standard normal variable transformation algorithms were used to pre-process the original hyperspectral data, which were then subjected to characteristic band extraction based on competitive adaptive reweighted sampling(CARS) and the partial least square regression analysis for building a quantitative model of SO_2 content in sulphur-fumigated Achyranthis Bidentatae Radix. It was found that the accuracy of the quantitative model built depending on the visible-near infrared spectra was high, with the determination coefficient of prediction set(R■) reaching 0.900 1. The established quantitative model has enabled the rapid and non-destructive detection of SO_2 content in sulphur-fumigated Achyranthis Bidentatae Radix, which can serve as an effective supplement to the method described in Chinese Pharmacopeia.
