Comparative study on in vivo and in vitro permeability of Huoxue Zhitong gel patch and microemulsion gel.
10.19540/j.cnki.cjcmm.20210823.305
- Author:
Hua-Hua LI
1
;
Chang YANG
1
;
Yan-Ling YANG
1
;
Jia-Yi HUANG
1
;
Ying-Yin ZHU
1
;
Shou-Ying DU
1
;
Jie BAI
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
- Publication Type:Journal Article
- Keywords:
Huoxue Zhitong gel patch;
eugenol;
microemulsion gel;
paeonol;
permeability in vitro;
pharmacokinetics in vivo;
piperine
- MeSH:
Administration, Cutaneous;
Chromatography, Liquid;
Emulsions;
Permeability;
Skin/metabolism*;
Skin Absorption;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2022;47(7):1857-1863
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to establish a method for determination of paeonol(Pae), eugenol(Eug), and piperine(Pip) content in receptor liquid and research on the permeability and pharmacokinetics of Huoxue Zhitong gel patch and microemulsion gel. The Franz diffusion experiment was conducted to assess the percutaneous permeability, and the microdialysis method was employed to assess pharmacokinetics of Huoxue Zhitong gel patch and microemulsion gel. The content of Pae, Eug, and Pip in receptor liquid in vitro and in vivo was determined by HPLC and UPLC-MS. The Q_n and J_(ss) of Pae, Eug, and Pip in the gel patch were significantly higher than those in the microemulsion gel, indicating that the drug release was faster in the gel patch. The C_(max), AUC_(0-760), and MRT of Pae, Eug, and Pip in the gel patch were higher than those in the microemulsion gel, indicating that the gel patch can promote the penetration and prolong the skin residence of the drug. The results of this study provide reference for improving the dosage form of Huoxue Zhitong patch.
