Complete chloroplast genome of Ligustrum lucidum and highly variable marker identification for Ligustrum.
10.19540/j.cnki.cjcmm.20220116.103
- Author:
Yuan-Xi JIN
1
;
Yu-Shuang WANG
1
;
Yong-Wei GAO
1
;
Li-Wei ZHOU
1
;
Yi-Heng WANG
2
;
Qing-Jun YUAN
2
;
Wen-Pan DONG
1
Author Information
1. School of Ecology and Nature Conservation, Beijing Forestry University Beijing 100083, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
DNA barcoding;
Ligustrum lucidum;
chloroplast genome;
phylogeny
- MeSH:
Fruit;
Genome, Chloroplast;
Ligustrum/genetics*;
Phylogeny;
Plant Breeding
- From:
China Journal of Chinese Materia Medica
2022;47(7):1847-1856
- CountryChina
- Language:Chinese
-
Abstract:
Ligustri Lucidi Fructus, the sun-dried mature fruit of Ligustrum lucidum, is cool, plain, sweet, and bitter, which can be used as both food and medicine, with the effects of improving vision, blacking hair, and tonifying liver and kidney. It takes effect slowly. However, little is known about the genetic information of the medicinal plant and it is still a challenge to distinguish Ligustrum species. In this study, the complete chloroplast genome of L. lucidum was obtained by genome skimming and then compared with that of five other Ligustrum species, which had been reported. This study aims to evaluate the interspecific variation of chloroplast genome within the genus and develop molecular markers for species identification of the genus. The result showed that the chloroplast genome of L. lucidum was 162 162 bp with a circular quadripartite structure of two single-copy regions separated by a pair of inverted repeats. The Ligustrum chloroplast genomes were conserved with small interspecific difference. Comparative analysis of six Ligustrum chloroplast genomes revealed three variable regions(rbcL-accD, ycf1a, and ycf1b), and ycf1a and ycf1b can be used as the species-specific DNA barcode for Ligustrum. Phylogeny analysis provided the best resolution of Ligustrum and supported that L. lucidum was sister to L. gracile. This study clarified the genetic diversity of L. lucidum from provenance, which can serve as a reference for further analysis of pharmacological differences and breeding of excellent varieties with stable drug effects.
