Identification and biological characteristics of a pathogen causing leaf blight of Rehmannia glutinosa.
10.19540/j.cnki.cjcmm.20220111.101
- Author:
Yu-Biao CAI
1
;
Tao DOU
1
;
Fu-Tao GAO
1
;
Jia-Fang DU
1
;
Feng-Qing WANG
1
;
Wen-Kai NIAN
1
;
Mo WANG
2
;
Zhong-Yi ZHANG
3
;
Xiang-Yang LIU
1
Author Information
1. Henan Agricultural University Zhengzhou 450002,China.
2. College of Plant Science and Technology,Huazhong Agricultural University Wuhan 430070,China.
3. College of Agriculture,Fujian Agriculture and Forestry University Fuzhou 350002,China.
- Publication Type:Journal Article
- Keywords:
Fusarium acuminatum;
Rehmannia glutinosa;
biological characteristics;
disease;
pathogen
- MeSH:
Carbon;
Nitrogen;
Phylogeny;
Rehmannia
- From:
China Journal of Chinese Materia Medica
2022;47(7):1824-1830
- CountryChina
- Language:Chinese
-
Abstract:
Leaf blight outbroke in Rehmannia glutinosa plantation in Wenxian county, Henan province in 2019. R. glutinosa plants with diseased leaves were collected from the plantation, and three strains were isolated from the diseased leaf samples. Pathogenicity test, morphological observation, and phylogenetic analysis of ITS, EF1-α, and Tub suggested that they were respectively Fusarium proliferatum, F. oxysporum, and F.acuminatum. Among them, F. acuminatum, as a pathogen of R. glutinosa leaf disease, had never been reported. To clarify the biological characteristics of F. acuminatum, this study tested the influence of light, pH, temperature, medium, carbon source, and nitrogen source on the mycelial growth rate of the pathogen during a 5-day culture period, and explored the lethal temperature. The results showed that the mycelia grew well under the photoperiod of 12 h light/12 h darkness, at 5-40 ℃(optimal temperature: 25 ℃), at pH 4-11(optimal pH: 7.0), on a variety of media(optimal medium: oatmeal agar), and in the presence of diverse carbon and nitrogen sources(optimal carbon source: soluble starch; optimal nitrogen source: sodium nitrate). The lethal temperature was verified to be 51 ℃(10 min). The conclusion is expected to lay a scientific basis for diagnosis and control of R. glutinosa leaf diseases caused by F. acuminatum.
