Identification of Q-markers for Cistanches Herba based on HPLC-Q-TOF-MS/MS and network pharmacology.
10.19540/j.cnki.cjcmm.20211210.201
- Author:
Zhao-Yuan CAO
1
;
Jian-Ting LIU
2
;
Yan-Qi HAN
2
;
Tie-Jun ZHANG
3
;
Jun XU
3
Author Information
1. Tianjin University of Traditional Chinese Medicine Tianjin 300193, China.
2. Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research Tianjin 300301, China.
3. Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research Tianjin 300301, China State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research Tianjin 300301, China.
- Publication Type:Journal Article
- Keywords:
Cistanches Herba;
HPLC-Q-TOF-MS/MS;
network pharmacology;
pharmacological mechanism;
quality markers(Q-markers);
traditional efficacy
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Cistanche;
Drugs, Chinese Herbal/pharmacology*;
Network Pharmacology;
Tandem Mass Spectrometry/methods*
- From:
China Journal of Chinese Materia Medica
2022;47(7):1790-1801
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to establish a method for analyzing the chemical constituents in Cistanches Herba by high performance liquid chromatography(HPLC) and quadrupole-time-of-flight tandem mass spectrometry(HPLC-Q-TOF-MS/MS), and to reveal the pharmacological mechanism based on network pharmacology for mining the quality markers(Q-markers) of Cistanches Herba. The chemical constituents of Cistanche deserticola and C. tubulosa were analyzed via HPLC-Q-TOF-MS/MS. The potential targets and pathways of Cistanches Herba were predicted via SwissTargetPrediction and DAVID. The compound-target-pathway-pharmacological action-efficacy network was constructed via Cytoscape. A total of 47 chemical constituents were identified, involving 95 targets and 56 signaling pathways. We preliminarily elucidated the pharmacological mechanisms of echinacoside, acteoside, isoacteoside, cistanoside F, 2'-acetylacteoside, cistanoside A, campneoside Ⅱ, salidroside, tubuloside B, 6-deoxycatalpol, 8-epi-loganic acid, ajugol, bartsioside, geniposidic acid, and pinoresinol 4-O-β-D-glucopyranoside, and predicted them to be the Q-markers of Cistanches Herba. This study identified the chemical constituents of Cistanches Herba, explained the pharmacological mechanism of the traditional efficacy of Cistanches Herba based on network pharmacology, and introduced the core concept of Q-markers to improve the quality evaluation of Cistanches Herba.