Evaluation of genetic diversity of ginseng fruit color germplasm resources: based on SSR analysis.
10.19540/j.cnki.cjcmm.20220115.102
- Author:
Ping QIAO
1
;
Yi-Heng WANG
2
;
Jia-Hui SUN
2
;
Chuan-Zhi KANG
2
;
Lan-Ping GUO
2
;
Lu-Qi HUANG
1
Author Information
1. Academician Workstation, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
SSR;
genetic diversity;
germplasm resources;
ginseng fruit color
- MeSH:
Fruit/genetics*;
Genetic Variation;
Microsatellite Repeats;
Panax/genetics*;
Plant Breeding
- From:
China Journal of Chinese Materia Medica
2022;47(8):2158-2164
- CountryChina
- Language:Chinese
-
Abstract:
Illumina Xten was employed for shallow sequencing of Panax ginseng(ginseng) samples, MISA for screening of SSR loci, and Primer 3 for primer design. Polymorphic primers were screened from 180 primers. From the successfully amplified polymorphic primers, 15 primers which featured clear peak shape, good polymorphism, and ease of statistics were selected and used to evaluate the genetic diversity and germplasm resources of 36 ginseng accessions with different fruit colors from Jilin province. The results showed that red-fruit ginseng population had high genetic diversity with the average number of alleles(N_a) of 1.031 and haploid genetic diversity(h) of 0.172. The neighbor-joining cluster analysis demonstrated that the germplasms of red-fruit and yellow-fruit ginseng populations were obviously intermixed, and pick-fruit ginseng germplasms clustered into a single clade. The results of STRUCTURE analysis showed high proportion of single genotype in pick-fruit ginseng germplasm and abundant genotypes in red-fruit and yellow-fruit ginseng germplasms with obvious germplasm mixing. AMOVA revealed that genetic variation occurred mainly within populations(62.00%, P<0.001), and rarely among populations(39%, P<0.001), but homogenization was obvious among different populations. In summary, pink-fruit ginseng population may contain rare genotypes, which is the basis for breeding of high-quality high-yield, and multi-resistance varieties, genetic improvement of varieties, and sustainable development and utilization of ginseng germplasm resources.
