UPLC-Q-TOF-MS analysis of chemical constituents of classical prescription Yiguanjian standard decoction.
10.19540/j.cnki.cjcmm.20211214.302
- Author:
Fu-Yan YANG
1
;
Ru-Ling XU
1
;
Wei NIU
2
;
Jie-Ge HUO
2
;
Jian-Ming JU
2
Author Information
1. School of Pharmacy, Anhui University of Traditional Chinese Medicine Hefei 230031, China Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028, China.
2. Jiangsu Province Academy of Traditional Chinese Medicine Nanjing 210028, China Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210028, China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS;
Yiguanjian;
chemical constituents;
classic prescription
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Flavonoids/analysis*;
Formates;
Glycosides/analysis*;
Prescriptions
- From:
China Journal of Chinese Materia Medica
2022;47(8):2134-2147
- CountryChina
- Language:Chinese
-
Abstract:
An UPLC-Q-TOF-MS method was employed to characterize and classify the chemical components of the standard decoction of Yiguanjian, a classical famous recipe. Chromatographic separation was performed on an Acquity HSS T3(2.1 mm ×100 mm, 1.8 μm) column with a mobile phase of 0.1% formic acid water-0.1% formic acid acetonitrile using gradient elution. The flow rate was 0.4 mL·min~(-1) and the column temperature was 40 ℃. Mass spectrometry was performed on electrospray ionization source(ESI) with positive and negative ion scanning modes. The potential compounds were identified by comparing the reference compounds, analyzing the mass spectrometry data and matching the published articles on Masslynx 4.1 software and SciFinder database. Finally, a total of 113 compounds, including 11 amino acids, 19 terpenoids, 13 phthalides, 11 steroidal saponins, 10 coumarins, 9 alkaloids, 7 flavonoids, 8 phenylethanoid glycosides, 8 organic acids and 17 other categories were identified. The established method systematically and accurately characterized the chemical components in Yiguanjian, which could provide experimental evidences for the subsequent studies on the pharmacodynamical material basis and quality control of Yiguanjian.
