Characteristic chromatogram and index components content of substance benchmark of Xuanfu Daizhe Decoction.
10.19540/j.cnki.cjcmm.20210604.301
- Author:
Hui XIE
1
;
Li LIN
1
;
Huan-Huan LI
1
;
Jing MAO
1
;
Jin-Guo XU
1
;
Chun-Qin MAO
1
;
Peng LI
2
;
Yin-Hong SHEN-TU
3
;
Tu-Lin LU
1
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. Nanjing Hailing of Traditional Chinese Medicine Pharmaceutical Technology Research Co., Ltd. Nanjing 210023, China.
3. Zhejiang Tongjuntang Chinese Medicine Decoction Pipe Co., Ltd. Tonglu 311502, China.
- Publication Type:Journal Article
- Keywords:
Xuanfu Daizhe Decoction;
characteristic chromatogram;
content determination;
substance benchmark
- MeSH:
Benchmarking;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Ginsenosides/analysis*;
Glycyrrhizic Acid/analysis*;
Quality Control
- From:
China Journal of Chinese Materia Medica
2022;47(8):2090-2098
- CountryChina
- Language:Chinese
-
Abstract:
The methods for determining the characteristic chromatogram and index components content of Xuanfu Daizhe Decoction were established to provide a scientific basis for the quality evaluation of substance benchmarks and preparations. Eighteen batches of Xuanfu Daizhe Decoction were prepared with the decoction pieces of different batches and of the same batch were prepared respectively, and the HPLC characteristic chromatograms of these samples were established. The similarities of the chromatographic fingerprints were analyzed. With liquiritin, glycyrrhizic acid, 6-gingerol, ginsenoside Rg_1, and ginsenoside Re as index components, the high performance liquid chromatography was established for content determination with no more than 70%-130% of the mass average as the limit. The results showed that there were 19 characteristic peaks corresponding to the characteristic chromatograms of 18 batches of Xuanfu Daizhe Decoction, including 8 peaks representing liquiritin, 1,5-O-dicaffeoylqunic acid, ginsenoside Rg_1, ginsenoside Re, 1-O-acetyl britannilactone, ginsenoside Rb_1, glycyrrhizic acid, and 6-gingerol, and the fingerprint similarity was greater than 0.97. The contents of liquiritin, glycyrrhizic acid, 6-gingerol, and ginsenosides Rg_1 + Re in the prepared Xuanfu Daizhe Decoction samples were 0.53%-0.86%, 0.61%-1.2%, 0.023%-0.068%, and 0.33%-0.66%, respectively. Except for several batches, most batches of Xuanfu Daizhe Decoction showed stable contents of index components, with no discrete values. The characteristic chromatograms and index components content characterized the information of Inulae Flos, Ginseng Radix et Rhizoma, Glycyrrhizae Radix et Rhizoma, and Zingiberis Rhizoma Recens in Xuanfu Daizhe Decoction. This study provides a scientific basis for the further research on the key chemical properties of substance benchmark and preparations of Xuanfu Daizhe Decoction.
