Serum metabolomics of Tibetan medicine Ershiwuwei Songshi Pills against chronic cholestasis in mice.
10.19540/j.cnki.cjcmm.20211204.702
- Author:
A-Rong LI
1
;
Cun-Ping WANG
1
;
Yi DING
1
;
Cheng-Fang JIAN
1
;
Le ZHANG
1
;
Jian GU
1
;
Rui TAN
2
Author Information
1. College of Pharmacy, Southwest Minzu University Chengdu 610041, China.
2. College of Life Science and Engineering, Southwest Jiaotong University Chengdu 610031, China.
- Publication Type:Journal Article
- Keywords:
Ershiwuwei Songshi Pills;
LC-MS;
cholestasis;
metabonomics
- MeSH:
Animals;
Bile Acids and Salts;
Cholestasis/drug therapy*;
Chromatography, Liquid;
Medicine, Tibetan Traditional;
Metabolomics;
Mice
- From:
China Journal of Chinese Materia Medica
2022;47(8):2056-2063
- CountryChina
- Language:Chinese
-
Abstract:
A chronic cholestasis model was induced in mice by feeding a diet containing 3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-trimethylpyridine(DDC). The effects of Ershiwuwei Songshi Pills(ESP) on endogenous metabolites in mice with chronic cholestasis were investigated by metabolomics analysis based on liquid chromatography-mass spectrometry(LC-MS). The results showed that ESP was effective in improving pathological injury and reducing serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP), and total bile acid in the model mice. Meanwhile, 13 common differential metabolites were revealed in metabolomic screening between the model/control group and the model/ESP group, including uric acid, glycolaldehyde, kynurenine, flavin adenine dinucleotide, L-3-phenyllactic acid, I-urobilin, leukotriene D4(LTD4), taurocholic acid, trioxilin A3, D-inositol-1,4-diphosphate, PC [16:0/20:2(11Z,14Z)], PC[14:0/22:2(13Z,16Z)], and PC[20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z)]. After ESP intervention, the levels of all 13 differential metabolites were significantly retraced, and pathway analysis showed that ESP achieved its therapeutic effect mainly by affecting arachidonic acid metabolism, glycerophospholipid metabolism, tryptophan metabolism, and primary bile acid biosynthesis. This study elucidated the mechanism of action of ESP against chronic cholestasis based on metabolites.
