Butyl alcohol extract of Baitouweng Decoction alleviates vulvovaginal candidiasis in mice by downregulating NLRP3 inflammasome and related signal pathways.
10.19540/j.cnki.cjcmm.20211223.701
- Author:
Kang ZHANG
1
;
Ting ZHAO
1
;
Dan XIA
1
;
Meng-Xiang ZHANG
1
;
Da-Qiang WU
1
;
Tian-Ming WANG
1
;
Jing SHAO
1
;
Chang-Zhong WANG
2
Author Information
1. School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine Hefei 230012, China.
2. School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine Hefei 230012, China Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine Hefei 230012, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui University of Chinese Medicine Hefei 230012, China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
Dectin-1/Sky;
NLRP3 inflammasome;
TLRs/MyD88;
butyl alcohol extract of Baitouweng Decoction;
vulvovaginal candidiasis
- MeSH:
1-Butanol/therapeutic use*;
Animals;
Candida albicans;
Candidiasis, Vulvovaginal/drug therapy*;
Caspase 1/metabolism*;
Cytokines/metabolism*;
Female;
Humans;
Inflammasomes/metabolism*;
Mice;
Myeloid Differentiation Factor 88/metabolism*;
NF-kappa B/metabolism*;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
Plant Extracts/therapeutic use*;
Reactive Oxygen Species/metabolism*;
Signal Transduction;
Toll-Like Receptor 2/metabolism*;
Toll-Like Receptor 4/metabolism*;
Tumor Necrosis Factor-alpha/metabolism*
- From:
China Journal of Chinese Materia Medica
2022;47(9):2516-2524
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to explore the effect of butyl alcohol extract of Baitouweng Decoction(BAEB) on vulvovaginal candidiasis(VVC) in mice and to clarify the mechanism from Toll-like receptors(TLRs)/MyD88 and Dectin-1/Syk signal pathways and NLRP3 inflammasome. To be specific, female KM mice were randomized into control group(i.g., normal saline), model group, fluco-nazole group(i.g., 20 mg·kg~(-1)), and low-dose, medium-dose, and high-dose BAEB groups(i.g., 20, 40, and 80 mg·kg~(-1), respectively). VVC was induced in mice except the control group. After the modeling, administration began and lasted 7 days. The ge-neral conditions and body weight of mice were recorded every day. On the 1 st, 3 rd, 7 th, and 14 th after vaginal infection by Candida albicans, the fungal load in the vaginal lavage fluid of the mice was measured with the plate method, and the morphology of C. albicans in vaginal lavage fluid was observed based on Gram staining. After the mice were killed, vaginal tissues were subjected to hematoxylin-eosin(HE) staining and periodic acid-Schiff(PAS) staining for vaginal histopathological analysis. The content of cytokines in vaginal lavage fluid, such as interleukin(IL)-1β, IL-18, tumor necrosis factor-α(TNF-α), IL-6, and S100 a8, was determined by enzyme-linked immunosorbent assay(ELISA), and content of reactive oxygen species(ROS) in vaginal tissues by tissue ROS detection kit. The protein expression of NLRP3, ASC, caspase-1, Dectin-1, Syk, MyD88, TLR2, TLR4, and nuclear factor-κB(NF-κB) in vaginal tissues was detected by Western blot, and the levels and distribution of NLRP3, Dectin-1, Syk, MyD88, TLR2, and TLR4 in vaginal tissues were determined with the immunohistochemical method. The results show that BAEB can improve the general conditions of VVC mice, reduce the fungal load and C. albicans hyphae in vaginal secretion, decrease ROS content in vaginal tissues and content of cytokines in vaginal lavage fluid, and down-regulate the expression of NLRP3, ASC, caspase-1, Dectin-1, Syk, MyD88, TLR2, TLR4, and NF-κB in vaginal tissues. The above results indicate that BAEB exerts therapeutic effect on VVC mice by down-regulating the key proteins in the TLRs/MyD88 and Dectin-1/Syk signal pathways and NLRP3 inflammasome.
