A new phloroglucinol from Dryopteris fragrans and its antibacterial activity in vitro.
10.19540/j.cnki.cjcmm.20220124.201
- Author:
Shi-Qian ZHENG
1
;
Guo-Qiang SONG
1
;
Chuan-Ping YIN
1
;
Yan-Fen CHEN
1
;
Shuai-Shuai WANG
2
;
Zhi-Bin SHEN
3
Author Information
1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 511400, China.
2. Guangzhou Hipower Pharmaceutical R&D Co., Ltd. Guangzhou 510620, China.
3. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 511400, China Guangdong Provincial Engineering Technology Research Center of Localized Precision Drug Delivery Preparation Guangzhou 510006, China Cosmetics Engineering Technology Research Center of Guangdong Province Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
Dryopteris fragrans;
antibacterial activity;
disaspidin BB;
phloroglucinol derivatives
- MeSH:
Anti-Bacterial Agents/pharmacology*;
Dryopteris;
Methicillin-Resistant Staphylococcus aureus;
Microbial Sensitivity Tests;
Phloroglucinol/pharmacology*;
Plant Extracts/pharmacology*
- From:
China Journal of Chinese Materia Medica
2022;47(9):2474-2479
- CountryChina
- Language:Chinese
-
Abstract:
A new phloroglucinol was isolated from 50% ethanol extract of Dryopteris fragrans by silica gel column chromatography, Sephadex LH-20 gel column chromatography, thin-layer chromatography(TLC), and preparative liquid column chromatography. On the basis of MS, ~1H-NMR, ~(13)C-NMR, and reference materials, compound 1 was identified as 2,5-cyclohexadien-1-one, 2-{[2,6-dihydroxy-4-methoxy-3-methyl-5-(1-isobutyl)phenyl]methyl}-3,5-dihydroxy-4,4-dimethyl-6-(1-oxobutyl)(1), and named disaspidin BB. Compound 1 was evaluated for its antibacterial activity. The experimental results showed that compared with the commonly used topical antibiotics erythromycin or mupirocin, disaspidin BB exhibited significant antibacterial activities against Staphylococcus epidermidis(SEP), S. haemolyticus(SHA), and methicillin-resistant S. aureus(MRSA)(P<0.05). Additionally, disaspidin BB was sensitive to ceftazidime-resistant SEP1-SEP4, SHA5-SHA7, MRSA8, and MRSA9. The MIC values of disaspidin BB against SEP and SHA were 1.67-2.71 μg·mL~(-1) and 10.00-33.33 μg·mL~(-1) respectively. Disaspidin BB has good antibacterial activities and deserves development as a new anti-infective drug for external use.
