Optimization of formulation of paclitaxel nanosuspension encapsulated by erythrocyte membrane based on Box-Behnken method.
10.19540/j.cnki.cjcmm.20210409.301
- Author:
Yue-Yue FAN
1
;
Yue-Xin CUI
2
;
Wen-Yan HAO
2
;
Meng-Yu CHEN
2
;
Yang YANG
2
;
Chun-Sheng GAO
1
Author Information
1. School of Pharmacy, Henan University Kaifeng 475000, China Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences Beijing 100850, China.
2. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences Beijing 100850, China.
- Publication Type:Journal Article
- Keywords:
Box-Behnken method;
erythrocyte membrane;
nanosuspension;
paclitaxel;
ultrasonic precipitation
- MeSH:
Erythrocyte Membrane;
Nanoparticles/chemistry*;
Paclitaxel/pharmacology*;
Particle Size;
Suspensions
- From:
China Journal of Chinese Materia Medica
2022;47(9):2457-2464
- CountryChina
- Language:Chinese
-
Abstract:
In view of the longevity and innate immune escape of red blood cells, this study designed the red blood cell membrane-coated paclitaxel nanosuspension [RBC-(PTX)NS] and investigated its physicochemical properties and antitumor effect in vitro. Paclitaxel nanosuspension [(PTX)NS] was prepared by ultrasonic precipitation and then RBC-(PTX)NS by ultrasonic coating. The formulation of(PTX)NS was optimized with Box-Behnken method and indexes of particle diameter, zeta potential, and stability. The morphology, particle diameter, stability, in vitro dissolution, and antitumor effect of(PTX)NS and RBC-(PTX)NS were characterized. The results showed that the particle diameter and zeta potential were(129.38±0.92) nm and(-22.41±0.48) mV, respectively, for the optimized(PTX)NS, while(142.5±0.68) nm and(-29.85±0.53) mV, respectively, for RBC-(PTX)NS. Under the transmission electron microscope,(PTX)NS was spherical and RBC-(PTX)NS had obvious core-shell structure. RBC-(PTX)NS remained stable for 5 days at 4 ℃. The in vitro dissolution test demonstrated that the cumulative release rate of RBC-(PTX)NS reached 79% within 20 min, which was significantly higher than that(25%) of(PTX)NS(P<0.05). As evidenced by MTT assay, RBC-(PTX)NS highly inhibited the proliferation of HepG2 cells in a dose-dependent manner. The cell membrane-coated nano-preparation preparation method is simple and reproducible. It improves the solubility of PTX and endows RBC-(PTX)NS with higher stability and stronger cytotoxicity. Thus, it is a new method for the delivery of PTX via nanocrystallization.
