Resistance of different ecotypes of Gastrodia elata to tuber rot.
10.19540/j.cnki.cjcmm.20220221.101
- Author:
Jin-Qiang ZHANG
1
;
Qing-Song YUAN
1
;
Zhen OUYANG
2
;
Cheng-Hong XIAO
1
;
Yuan WEI
2
;
Yan-Hong WANG
1
;
Jiao XU
1
;
Xin TANG
1
;
Sheng WANG
3
;
Xiao WANG
4
;
Tao ZHOU
1
Author Information
1. Guizhou University of Traditional Chinese Medicine Guiyang 550025, China.
2. Jiangsu University Zhenjiang 212013, China.
3. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
4. Shandong Analysis and Test Center Ji'nan 250014, China.
- Publication Type:Journal Article
- Keywords:
Gastrodia elata;
ecotypes;
resistance;
tuber rot
- MeSH:
Ecotype;
Gastrodia/microbiology*;
Gene Expression Profiling;
Plant Tubers/genetics*
- From:
China Journal of Chinese Materia Medica
2022;47(9):2281-2287
- CountryChina
- Language:Chinese
-
Abstract:
Tuber rot has become a serious problem in the large-scale cultivation of Gastrodia elata. In this study, we compared the resistance of different ecotypes of G. elata to tuber rot by field experiments on the basis of the investigation of G. elata diseases. The histological observation and transcriptome analysis were conducted to reveal the resistance differences and the underlying mechanisms among different ecotypes. In the field, G. elata f. glauca had the highest incidence of tuber rot, followed by G. elata f. viridis, and G. elata f. elata and G. elata f. glauca×G. elata f. elata showed the lowest incidence. Tuber rot showcased obvious plant source specificity and mainly occurred in the buds and bottom of G. elata plants. After infection, the pathogen spread hyphae in host cortex cells, which can change the endophytic fungal community structure in the cortex and parenchyma of G. elata. G. elata f. glauca had thinner lytic layer and more sugar lumps in the parenchyma than G. elata f. elata. The transcription of genes involved in immune defense, enzyme synthesis, polysaccharide synthesis, carbohydrate transport and metabolism, hydroxylase activity, and aromatic compound synthesis had significant differences between G. elata f. glauca and G. elata f. elata. These findings suggested that the differences in resis-tance to tuber rot among different ecotypes of G. elata may be related to the varied gene expression patterns and secondary metabolites. This study provides basic data for the prevention and control of tuber rot and the improvement of planting technology for G. elata.
