Effect of Kansui Radix stir-fried with vinegar on fecal metabolomics in Walker-256 rats with ascites.
10.19540/j.cnki.cjcmm.20210412.305
- Author:
Shi-Kang ZHOU
1
;
Dong-Jing JIANG
2
;
Yi ZHANG
1
;
Li ZHANG
1
Author Information
1. Jiangsu Provincial Collaborative Innovation Center for Industrialization Process of Traditional Chinese Medicine Resources/National-Local Joint Engineering Research Center for Traditional Chinese Medicine Resources Industrialization, Prescriptions and Innovative Drugs, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. School of Pharmacy, Suzhou Vocational Health College Suzhou 215000, China.
- Publication Type:Journal Article
- Keywords:
Kansui Radix stir-fried with vinegar;
fecal metabolomics;
malignant ascites;
metabolic pathway analysis
- MeSH:
Acetic Acid;
Animals;
Ascites/metabolism*;
Euphorbia;
Feces;
Metabolomics;
Rats
- From:
China Journal of Chinese Materia Medica
2022;47(6):1558-1566
- CountryChina
- Language:Chinese
-
Abstract:
Utilizing metabolomics technology, this study explored the change of fecal endogenous metabolites in Walker-256 rats with malignant ascites after the administration with Kansui Radix(KR) stir-fried with vinegar(VKR), sought the potential biomarkers in feces which were related to the treatment of malignant ascites by VKR and revealed the biological mechanism of water-expelling effect of VKR. Ultra-fast liquid chromatography-quadrupole-time-of-flight mass spectrometry(UFLC-Q-TOF-MS) was employed to detect the feces of rats in all groups. Principle component analysis(PCA) and partial least squares discriminant analysis(PLS-DA) were conducted to achieve pattern recognition. Combining t-test and variable importance in the projection(VIP) enabled the screening of potential biomarkers for the malignant ascites. Metabolic pathway analysis was accomplished with MetaboAnalyst. Correlation analysis was finally conducted integrating the sequencing data of gut microbiota to elucidate the mechanism underlying the water-expelling effect of VKR. The results showed that both KR and VKR could restore the abnormal metabolism of model rats to some extent, with VKR being inferior to KR in the regulation. Eleven potential biomarkers were identified to be correlated with the malignant ascites and five metabolic pathways were then enriched. Four kinds of gut microbiota were significantly related to the potential biomarkers. The water-expelling effect of VKR may be associated with the regulation of phenylalanine metabolism, biosynthesis of phenylalanine, tyrosine and tryptophan, tryptophan metabolism, glycerophospholipid metabolism, and glycosylphosphatidylinositol(GPI)-anchor biosynthesis. This study can provide a scientific basis for comprehensive understandings of the interaction between gut microbiota and host which has relation to the water-expelling effect of VKR and guide the reasonable clinical application of VKR.
