Acetylation of Rehmannia glutinosa polysaccharides and antioxidant activity of acetylated derivatives.
10.19540/j.cnki.cjcmm.20210310.302
- Author:
Jin LI
1
;
Ting-Ting ZHANG
1
;
Ding-Tao PU
1
;
Ya-Jun SHI
1
;
Zhen-Yu ZUO
1
;
Chong-Ying LIU
1
;
Yan CHEN
1
;
Xiao-Bin JIA
2
;
Peng ZHAO
1
;
Liang FENG
2
Author Information
1. College of Pharmacy, Shaanxi University of Chinese Medicine Xianyang 712046, China.
2. School of Traditional Chinese Pharmacy, China Pharmaceutical University Nanjing 211198, China.
- Publication Type:Journal Article
- Keywords:
Rehmannia glutinosa;
acetylation;
antioxidant;
polysaccharides;
response surface methodology
- MeSH:
Acetylation;
Antioxidants/pharmacology*;
Polysaccharides/pharmacology*;
Rehmannia/chemistry*
- From:
China Journal of Chinese Materia Medica
2022;47(6):1539-1545
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to acetylate Rehmannia glutinosa polysaccharides by acetic anhydride method, optimize process parameters and evaluate their antioxidant activity. With the degree of substitution(D_s) as a criterion, the effects of reaction time, acetic anhydride-to-polysaccharides ratio and temperature were investigated. Process parameters were optimized by single-factor experiment and response surface methodology. The infrared spectroscopy(IR) and scanning electron microscopy(SEM) proved the successful acetylation and were employed to preliminarily analyze the structural characteristics of acetylated derivatives. The results showed that the D_s was 0.327 under the optimal technological conditions, including m(acetic anhydride):m(R. glutinosa polysaccharides)=2.70, reaction time 3.0 h and temperature 48 ℃. Further, the antioxidant properties of acetylated derivatives were investigated in vitro and acetylation was found effective to improve the antioxidant activity of R. glutinosa polysaccharides. This study provides a reference for the further development and application of R. glutinosa polysaccharides.
