Study on critical quality attributes of Qingjin Huatan Decoction based on serum pharmacochemistry.
10.19540/j.cnki.cjcmm.20211122.201
- Author:
Jian-Ting LIU
1
;
Hong-Peng ZHAO
2
;
Qiang ZHU
3
;
Hong-Bing ZHANG
1
;
Xiang-Yu LI
3
;
Yan-Qi HAN
1
;
Quan WANG
3
;
Jun XU
4
;
Tie-Jun ZHANG
4
Author Information
1. Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research Tianjin 300301, China.
2. Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
3. Anhui Jiren Pharmaceutical Co., Ltd. Bozhou 236800, China.
4. Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine, Tianjin Institute of Pharmaceutical Research Tianjin 300301, China State Key Laboratory of Drug Release Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research Tianjin 300301, China.
- Publication Type:Journal Article
- Keywords:
Qingjin Huatan Decoction;
critical quality attributes;
serum pharmacochemistry
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Chromatography, Liquid;
Drugs, Chinese Herbal/chemistry*;
Tandem Mass Spectrometry/methods*
- From:
China Journal of Chinese Materia Medica
2022;47(5):1392-1402
- CountryChina
- Language:Chinese
-
Abstract:
Qingjin Huatan Decoction is a classic prescription with the effects of clearing heat, moistening lung, resolving phlegm, and relieving cough. In order to explore the critical quality attributes of Qingjin Huatan Decoction, we identified the blood components of Qingjin Huatan Decoction by ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS) under the following conditions, chromatographic column: Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm); mobile phase: 0.1% formic acid acetonitrile(A)-0.1% formic acid in water(B); gradient elution; flow rate: 0.2 mL·min~(-1); column temperature: 30 ℃; injection volume: 5 μL. The electrospray ionization(ESI) source was used to collect data in both positive and negative ion modes under the following conditions, capillary voltage: 3 kV for the positive ion mode and 2 kV for the negative ion mode; ion source temperature: 110 ℃; cone voltage: 30 V; cone gas flow rate: 50 L·h~(-1); nitrogen degassing temperature: 350 ℃; degassing volume flow rate: 800 L·h~(-1); scanning range: m/z 50-2 000. In this experiment, a total of 66 related components of Qingjin Huatan Decoction were identified, including 22 prototype components and 44 metabolites. The results of this study preliminarily revealed the pharmacodynamic material basis of Qingjin Huatan Decoction in vivo, which has provided an experimental basis for the determination of quality markers of Qingjin Huatan Decoction and the development of new drugs.
