Effect of Pien Tze Huang against EV71.
10.19540/j.cnki.cjcmm.20211117.701
- Author:
Jin-Xia LIN
1
;
Juan YU
1
;
Zhi-Liang CHEN
1
;
Shi-Cong WANG
1
;
Fei HONG
1
;
Yi-Chao ZHUANG
1
;
Wen-Liang LAN
1
Author Information
1. Fujian Provincial Key Laboratory of Pien Tze Huang Natural Medicine Research and Development, Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd. Zhangzhou 363000, China.
- Publication Type:Journal Article
- Keywords:
Pien Tze Huang;
antiviral effect;
enterovirus 71;
hand-foot-and-mouth disease
- MeSH:
Animals;
Chlorocebus aethiops;
Drugs, Chinese Herbal/pharmacology*;
Enterovirus A, Human/physiology*;
Hand, Foot and Mouth Disease;
Vero Cells
- From:
China Journal of Chinese Materia Medica
2022;47(5):1343-1349
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the inhibitory effect of Pien Tze Huang(PZH) on enterovirus 71(EV71). To be speci-fic, chemiluminescence method was adopted to evaluate the toxicity of PZH to African green monkey kidney(Vero) cells and human rhabdomyosarcoma(RD) cells, and cytopathic effect(CPE) method to assess the inhibition on EV71-GFP reporter virus and EV71 C4 wild-type virus. The results showed that PZH had low cytotoxicity to Vero cells and RD cells, with the half-maximal cytotoxic concentration(CC_(50)) of about 0.691 3-0.879 2 mg·mL~(-1) for the two. In addition, PZH can effectively inhibit the replication of EV71 within the non-cytotoxic concentration range, and dose-dependently alleviate the cytopathic changes caused by virus infection, with the half-maximal effective concentration(EC_(50)) of 0.009 2-0.106 3 mg·mL~(-1). On the basis of the above results, the green fluorescent protein(GFP), indirect immunofluorescence assay(IFA), and median tissue culture infective dose(TCID_(50)) were employed to assess and verify the anti-EV71-GFP and anti-EV71 C4 activity of PZH. The results demonstrated that PZH can dose-dependently lower the expression of GFP by EV71-GFP and structural protein VP-1 by EV71 C4 and decrease the production of progeny infectious viruses. The EC_(50) of PZH for EV71-GFP and EV71 C4 was about 0.006 0-0.006 2 mg·mL~(-1) and 0.006 6-0.025 6 mg·mL~(-1), respectively. This study suggested that PZH may exert antiviral activity by acting on EV71 and interfering with the expression of VP-1. At the moment, there is still a lack of specific anti-EV71 drugs. This study proposed a new idea for the symptomatic treatment of EV71 infections such as hand-foot-mouth disease and verified an effective drug for the treatment of EV71 infections.
