Spatial distribution characteristics of metabolities in rhizome of Paris polyphylla var. yunnanensis: based on MALDI-MSI.
10.19540/j.cnki.cjcmm.20211223.101
- Author:
Guan-Hua ZHANG
1
;
Xiao-Li LIU
2
;
Chun-Xia MA
3
;
Wen-Han LI
4
;
Xiao WANG
3
Author Information
1. College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine Kunming 650500, China Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences) Ji'nan 250014, China.
2. College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine Kunming 650500, China.
3. Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences) Ji'nan 250014, China.
4. School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences) Ji'nan 250353, China.
- Publication Type:Journal Article
- Keywords:
Paris;
Paris polyphylla var.yunnanensis;
matrix-assisted laser desorption/ionization-mass spectrometry imaging(MALDI-MSI);
metabolites;
tissue distribution
- MeSH:
Liliaceae/chemistry*;
Melanthiaceae;
Rhizome/chemistry*;
Saponins/analysis*;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From:
China Journal of Chinese Materia Medica
2022;47(5):1222-1229
- CountryChina
- Language:Chinese
-
Abstract:
In this study, a method was established for in-situ visualization of metabolite distribution in the rhizome of Paris polyphylla var. yunnanensis. To be specific, through matrix-assisted laser desorption/ionization-mass spectrometry imaging(MALDI-MSI), the spatial locations of steroidal saponins, amino acids, organic acids, phytosterols, phytoecdysones, nucleosides, and esters in rhizome of the medicinal plant were directly analyzed, and six unknown compounds with differential distribution in rhizome tissues were identified. The specific procedure is as follows: preparation of rhizome tissue section, matrix screening and optimization, and MALDI-MSI analysis. The results showed that the steroidal saponins were mainly distributed in the central, amino acids in epidermis and cortex, low-molecular-weight organic acids in central epidermis, phytosterols in the epidermis and lateral cortex, the phytoecdysones in epidermis and cortex, nucleosides(uneven distribution) in epidermis and cortex, growth hormones around the epidermis and cortex, particularly outside the cortex, and esters in cortex with unobvious difference among different tissues. In this study, the spatial distribution of meta-bolites in the rhizome of P. polyphylla var. yunnanensis was characterized for the first time. The result can serve as a reference for identifying and extracting endogenous metabolites of P. polyphylla var. yunnanensis, exploring the synthesis and metabolism mechanisms of the metabolites, and evaluating the quality of medicinal materials.
