Identification, biological characteristics, and fungicide screening of pathogen of black spot in Aconitum carmichaelii.
10.19540/j.cnki.cjcmm.20211222.103
- Author:
Jia ZHOU
1
;
Tie-Lin WANG
2
;
Yu-Huan MIAO
1
;
Da-Hui LIU
1
Author Information
1. Resource Center of Chinese Materia Medica, Hubei University of Chinese Medicine Wuhan 430065, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Aconitum carmichaelii;
Alternaria alternata;
biological characteristics;
black spot;
fungicide screening
- MeSH:
Aconitum;
Alternaria;
Fungicides, Industrial/pharmacology*;
Mycelium
- From:
China Journal of Chinese Materia Medica
2022;47(5):1215-1221
- CountryChina
- Language:Chinese
-
Abstract:
In Hezhang county, Guizhou province, black spot tends to occur to Aconitum carmichaelii in the hot rainy summer, with the incidence up to 50%-70%, seriously impacting the yield and quality of the medicinal material. Thus, this study aims to clarify the pathogen and the occurrence characteristics. To be specific, the pathogen was isolated and identified according to Koch's postulates and the pathogenicity and biological characteristics were determined. In addition, the sensitivity of the pathogen to four microbial fungicides, four botanical fungicides, and five chemical fungicides was determined with the mycelium growth rate method for the purpose of screening out optimal fungicides. The pathogen was identified as Alternaria alternate, as evidenced by the similar colony morphology and microscopic characteristics and 99.55%-100% similarity in sequences of rDNA-ITS, LSU, 18S, and TEF of the two. The optimum growth conditions for A. alternata were 28 ℃, pH 8, and continuous darkness. Bacillus subtilis had strong inhibitory effect on the pathogen, and the inhibition rate was more than 90% when the concentration was 1 mg·L~(-1). In addition, difenoconazole and quinoline copper can also control the pathogen, with median effective concentration(EC_(50)) of 2.92 and 9.02 mg·L~(-1), respectively. This study lays a theoretical basis for the field control of black spot in A. carmichaelii.
