Mechanism of Tibetan medicine Pterocephalus hookeri extract in treatment of rheumatoid arthritis based on serum metabonomics.
10.19540/j.cnki.cjcmm.20211103.704
- Author:
Ce TANG
1
;
Zhi-Qiang GAN
2
;
Shi-Ying LUO
2
;
Juan YANG
2
;
Meng YU
3
;
Zhong-Mei ZOU
3
;
Yi ZHANG
1
Author Information
1. School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China Ethnic Medicine Academic Heritage Innovation Research Center, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
2. School of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Pterocephalus hookeri;
Tibetan medicine;
rheumatoid arthritis;
serum metabolomics;
ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)
- MeSH:
Animals;
Arthritis, Rheumatoid/drug therapy*;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/pharmacology*;
Medicine, Tibetan Traditional;
Metabolomics;
Rats
- From:
China Journal of Chinese Materia Medica
2022;47(4):1001-1008
- CountryChina
- Language:Chinese
-
Abstract:
Ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF/MS) was used to investigate the effect of Pterocephalus hookeri on serum metabolism of adjuvant arthritis(AA) model rats induced by complete Freund's adjuvant. After the AA model was properly induced, the serum of rats was collected 30 days after treatment. UPLC-Q-TOF-MS chromatograms were collected and analyzed by principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA). The results revealed that compared with the control group, the model group showed increased content of 12 biomarkers in the serum(P<0.05) and reduced content of the other nine biomarkers(P<0.05). P. hookeri extract could recover the above-mentioned 19 biomarkers to a certain range. Pathway enrichment showed that these markers mainly involved eight metabolic pathways, including valine, leucine, and isoleucine degradation, arachidonic acid metabolism, arginine and proline metabolism, glycerol phospholipid metabolism, primary bile acid biosynthesis, bile acid biosynthesis, tryptophan metabolism, and unsaturated fatty acid biosynthesis. The findings of this study demonstrate that P. hookeri extract can regulate metabolic disorders and promote the regression of metabolic phenotype to the normal level to exert the therapeutic effect on AA rats. This study is expected to provide a certain scientific basis for the biological research on the treatment of rheumatoid arthritis by P. hookeri.
