Comparison of chemical components between aerial and underground parts of Coptis chinensis based on UPLC-Q-TOF-MS~E technology.
10.19540/j.cnki.cjcmm.20211102.201
- Author:
Tong ZHU
1
;
Dan YANG
2
;
Shan-Shan LIU
3
;
Fei TENG
2
;
Jing-Jing ZHU
2
;
Yong-Xin ZHANG
2
;
Guo-Bing XU
4
;
Shou-Jin LIU
5
;
Zhi-Min WANG
2
;
Liang-Mian CHEN
2
;
Hui-Min GAO
2
Author Information
1. School of Pharmary, Anhui University of Chinese Medicine Hefei 230012, China National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Institute of Analysis and Testing, Beijing Academy of Science and Technology(Beijing Center for Physical and Chemical Analysis) Beijing 100089, China.
4. School of Pharmary, Anhui University of Chinese Medicine Hefei 230012, China Anhui Institute for Food and Drug Control Hefei 230051, China.
5. School of Pharmary, Anhui University of Chinese Medicine Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
Coptis chinensis;
mass spectrometry-based identification;
non-medicinal organs of Chinese medicinal herbs
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Coptis chinensis;
Spectrometry, Mass, Electrospray Ionization/methods*;
Tandem Mass Spectrometry/methods*;
Technology
- From:
China Journal of Chinese Materia Medica
2022;47(4):980-987
- CountryChina
- Language:Chinese
-
Abstract:
The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS~E) technology was employed to compare the chemical components between the aerial and underground parts of Coptis chinensis samples from different batches. According to the retention time, molecular ion peak, and LC-MS~E fragment information of the reference substances and available literature, we identified a total of 40 components. Thirty-three and 31 compounds were respectively identified in the underground part(taproots) and the aerial part(stems and leaves) of C. chinensis. Among them, 24 compounds, including alkaloids(e.g., berberine and jatrorrhizine) and phenolic acids(e.g., chlorogenic acid, quinic acid, and tanshinol), were common in the two parts. In addition, differential components were also identified, such as magnoline glucoside in the underground part and(±) lariciresionol-4-β-D-glucopyranoside in the aerial part. The analysis of fragmentation pathways based on spectra of reference substances indicated the differences among samples of different batches. Furthermore, we performed the principal component analysis(PCA) for the peak areas of C. chinensis in different batches. The results showed that the underground part and the aerial part were clearly clustered into two groups, indicating that the chemical components contained in the two parts were different. Furthermore, the results of partial least squares discriminant analysis(PLS-DA) identified 31 differential compounds(VIP value>1) between the underground part and the aerial part, mainly including alkaloids, phenolic acids, lignans, and flavonoids. This study proves that C. chinensis possesses great development potential with multiple available compounds in stems and leaves. Moreover, it sheds light on for the development and utilization of non-medicinal organs of C. chinensis and other Chinese medicinal herbs.
