Analysis of chemical constituents in ethyl acetate extract of Taxilli Herba by UPLC-Q-Exactive-MS and screening of potential xanthine oxidase inhibitors.
10.19540/j.cnki.cjcmm.20210914.201
- Author:
Yuan LIANG
1
;
Li LI
2
;
Yi CAI
1
;
Li-Ba XU
3
;
Feng-Feng XIE
3
;
Da-Long LIANG
1
;
Fu-Lai CHEN
1
Author Information
1. Guangxi University of Chinese Medicine Nanning 530200, China.
2. Guangxi University of Chinese Medicine Nanning 530200, China Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
3. Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine Nanning 530200, China.
- Publication Type:Journal Article
- Keywords:
Taxilli Herba;
UPLC-Q-Exactive-MS;
chemical constituent;
xanthine oxidase
- MeSH:
Acetates;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Tandem Mass Spectrometry;
Xanthine Oxidase
- From:
China Journal of Chinese Materia Medica
2022;47(4):972-979
- CountryChina
- Language:Chinese
-
Abstract:
The present study analyzed and identified the chemical constituents from ethyl acetate(EA) extract of Taxilli Herba with UPLC-Q-Exactive-MS and screened active xanthine oxidase(XO) inhibitors with HPLC. The analysis was performed on an Hypersil GOLD C_(18) reversed-phase column(2.1 mm×50 mm, 1.9 μm), with the mobile phase of water containing 1% formic acid(A) and methanol(B) under gradient elution, the flow rate of 0.3 mL·min~(-1), and the injection volume of 5 μL. ESI source was used for MS and the compounds were collected in positive and negative ion modes. Xcalibur 4.1 was used to analyze the retention time, accurate relative molecular weight, and fragmentation of the compounds. The inhibitory activity of some known compounds on XO was screened by HPLC. Thirty chemical constituents were identified, including phenolic acids and flavonoids by experimental data combined with information of standards, data reported previously, and databases, such as MzCloud and ChemSpider. The activities of 10 chemical components were screened. Gallic acid and naringenin chalcone had strong inhibitory activities on XO with IC_(50) of 57 μg·mL~(-1) and 108 μg·mL~(-1). UPLC-Q-Exactive-MS allows the accurate, rapid, and comprehensive identification of main chemical constituents from Taxilli Herba. Gallic acid and naringenin chalcone may be the active components of XO inhibitors.
