Contributions of flavonoids from citri reticulatae pericarpium to gastric hormones, CD3~+ and TFF3 mRNA expression in rats with spleen deficiency intervened by Liujunzi Decoction.
10.19540/j.cnki.cjcmm.20210901.301
- Author:
Shao-Wa LYU
1
;
Ying LI
1
;
Xin YU
1
;
Yu-Yan GUO
1
;
Da-Yu YANG
1
;
Shuang SUN
1
;
Er-Yu SHANG
2
Author Information
1. Heilongjiang Province Key Laboratory of Basic and Applied Research of Northern Medicine,Ministry of Education,Heilongjiang Province Key Laboratory of Basic Research of Pharmacodynamic Substances of Traditional Chinese Medicine and Natural Medicine,Heilongjiang University of Chinese Medicine Harbin 150040, China.
2. Heilongjiang Institute of Forest Protection Harbin 150040, China.
- Publication Type:Journal Article
- Keywords:
Citri Reticulatae Pericarpium;
Liujunzi Decoction;
fingerprint;
gray correlation analysis;
spectrum-effect relationship
- MeSH:
Animals;
Citrus/chemistry*;
Drugs, Chinese Herbal;
Flavonoids/pharmacology*;
Hormones;
RNA, Messenger/genetics*;
Rats;
Spleen
- From:
China Journal of Chinese Materia Medica
2022;47(4):951-958
- CountryChina
- Language:Chinese
-
Abstract:
The present study established the spectrum-effect relationship model of flavonoids in Citri Reticulatae Pericarpium(CRP) from 15 batches of Liujunzi Decoction and statistically analyzed the correlation between chemical peaks and efficacy to identify the main effective components. HPLC fingerprints of flavonoids in CRP from 15 batches of Liujunzi Decoction were established. HPLC analysis was carried out on the Venusil XBP C_(18)(L) column(4.6 mm×250 mm, 5 μm) at 30 ℃ with acetonitrile-water(containing 0.1% formic acid) as mobile phase for gradient elution, a flow rate of 1.0 mL·min~(-1), and detection wavelength of 300 nm to obtain chemical fingerprints. Additionally, the effects of flavonoids from CRP in 15 batches of Liujunzi Decoction on the content of GAS, MTL, and VIP, TFF3 mRNA expression, and percentage of CD3~+ T-cells of model rats with spleen deficiency were determined. The spectrum-effect relationship model was established by gray correlation analysis. The results showed that the main characteristic peaks with great contribution to the regulation of gastrointestinal tract were peak 16(vicenin-2), peak 63(sinensetin), peak 64(isosinensetin), peak 65(nobiletin), peak 67(3,5,6,7,8,3',4'-heptemthoxyflavone), peak 68(tangeretin), and peak 69(5-desmethylnobiletin). Therefore, there was a linear correlation between flavonoids from CRP in Liujunzi Decoction and the efficacy, and the medicinal effect was achieved by multi-component action. This study is expected to provide a new idea for exploring the material basis of the effect, i.e., regulating qi prior to replenishing qi, of CRP in Liujunzi Decoction.
