Dynamic control of ERG20 expression to improve production of monoterpenes by engineering Saccharomyces cerevisiae.
10.19540/j.cnki.cjcmm.20210913.101
- Author:
Rong-Sheng LI
1
;
Dong WANG
2
;
Yu-Song SHI
2
;
Li-Ping XU
2
;
Xue-Li ZHANG
2
;
Kou WANG
3
;
Zhu-Bo DAI
2
Author Information
1. School of Pharmacy and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University Kunming 650500, China Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences Tianjin 300308,China Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences Tianjin 300308, China.
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences Tianjin 300308,China Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences Tianjin 300308, China.
3. School of Pharmacy and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University Kunming 650500, China.
- Publication Type:Journal Article
- Keywords:
ERG20;
Saccharomyces cerevisiae;
biosynthesis;
monoterpenes
- MeSH:
Fermentation;
Geranyltranstransferase/genetics*;
Monoterpenes/metabolism*;
Saccharomyces cerevisiae/metabolism*;
Saccharomyces cerevisiae Proteins/metabolism*
- From:
China Journal of Chinese Materia Medica
2022;47(4):897-905
- CountryChina
- Language:Chinese
-
Abstract:
Monoterpenes are widely used in cosmetics, food, medicine, agriculture and other fields. With the development of synthetic biology, it is considered as a potential way to create microbial cell factories to produce monoterpenes. Engineering Saccharomyces cerevisiae to produce monoterpenes has been a research hotspot in synthetic biology. In S. cerevisiae, the production of geranyl pyrophosphate(GPP) and farnesyl pyrophosphate(FPP) is catalyzed by a bifunctional enzyme farnesyl pyrophosphate synthetase(encoded by ERG20 gene) which is inclined to synthesize FPP essential for yeast growth. Therefore, reasonable control of FPP synthesis is the basis for efficient monoterpene synthesis in yeast cell factories. In order to achieve dynamic control from GPP to FPP biosynthesis in S. cerevisiae, we obtained a novel chassis strain HP001-pERG1-ERG20 by replacing the ERG20 promoter of the chassis strain HP001 with the promoter of cyclosqualene cyclase(ERG1) gene. Further, we reconstructed the metabolic pathway by using GPP and neryl diphosphate(NPP), cis-GPP as substrates in HP001-pERG1-ERG20. The yield of GPP-derived linalool increased by 42.5% to 7.6 mg·L~(-1), and that of NPP-derived nerol increased by 1 436.4% to 8.3 mg·L~(-1). This study provides a basis for the production of monoterpenes by microbial fermentation.
