Comparative study of rat serum pharmacochemistry between Puerariae Lobatae Radix and Puerariae Thomsonii Radix based on UPLC-Q-TOF-MS.
10.19540/j.cnki.cjcmm.20210804.201
- Author:
Hong-Hua YU
1
;
Xiao-Wei MENG
1
;
Jia-Rong LI
1
;
Hui OUYANG
2
;
Qian-Qian YIN
1
;
Li-Hua LIN
1
;
Wei-Feng ZHU
3
;
Rong-Hua LIU
1
Author Information
1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
2. National Engineering Research Center for Manufacturing Technology of Solid Preparations of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
3. Key Laboratory of Modern Preparations of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
- Publication Type:Journal Article
- Keywords:
Puerariae Lobatae Radix;
Puerariae Thomsonii Radix;
UPLC-Q-TOF-MS;
metabolites;
prototype components;
serum pharmacochemistry
- MeSH:
Animals;
Drugs, Chinese Herbal;
Plant Roots;
Pueraria;
Rats;
Serum
- From:
China Journal of Chinese Materia Medica
2022;47(2):528-536
- CountryChina
- Language:Chinese
-
Abstract:
UPLC-Q-TOF-MS and serum pharmacochemistry were employed to study the migrating components in rat sera after intragastric administration of the water extracts of Puerariae Lobatae Radix(PLR) and Puerariae Thomsonii Radix(PTR). After the respective intragastric administration of PLR and PTR extracts, blood samples were collected from the orbital vein. The serum samples were treated by protein precipitation method with methanol and acetonitrile at a ratio of 1∶1 and then passed through Agilent ZORBAX RRHD SB-C_(18) column(3 mm×100 mm, 1.8 μm) and Agilent SB-C_(18) pre-column(3 mm×5 mm, 1.8 μm) with 0.1% formic acid aqueous solution(A)-acetonitrile(B) as the mobile phase. The elution was performed at the flow rate of 0.25 mL·min~(-1), the column temperature of 40 ℃, and the injection volume of 2 μL. By comparison of the total ion chromatogram and secondary fragment ion information of PLR and PTR water extracts, PLR-and PTR-containing sera, and blank serum, we found 42 migrating components(including 17 prototype components and 25 metabolites) in the sera of rats treated with PLR and 35 migrating components(including 15 prototype components and 20 metabolites) in the sera of rats treated with PTR. Thirty-three common components were shared by the two treatments, including 13 prototype components and 20 metabolites. The differences of migrating components in the PLR-and PTR-treated rat sera provide a scientific basis for further study of the active components and quality markers of PLR and PTR.
