Multi-index components of Scutellariae Barbatae Herba according to UHPLC-QTRAP-MS coupled with chemometrics.
10.19540/j.cnki.cjcmm.20210901.201
- Author:
Jia-Lu JIANG
1
;
An KANG
1
;
Qin LI
1
;
Meng-Meng SONG
1
;
Hui-Fa HAN
1
;
Yu-Qing CAI
1
;
Zhui-Cheng XU
1
;
Hai-Bo CHENG
2
;
Dong-Dong SUN
3
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. Collaborative Innovation Center of Jiangsu Province of Cancer Prevention and Treatment of Chinese Medicine Nanjing 210023, China.
3. Collaborative Innovation Center of Jiangsu Province of Cancer Prevention and Treatment of Chinese Medicine Nanjing 210023, China School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
HCA;
PLS-DA;
Scutellariae Barbatae Herba;
UHPLC-QTRAP-MS
- MeSH:
Chemometrics;
Chromatography, High Pressure Liquid/methods*;
Chromatography, Liquid;
Scutellaria;
Tandem Mass Spectrometry/methods*
- From:
China Journal of Chinese Materia Medica
2022;47(2):437-443
- CountryChina
- Language:Chinese
-
Abstract:
The present study developed an ultra-fast liquid chromatography coupled with triple quadrupole-linear ion trap composite mass spectrometry(UHPLC-QTRAP-MS) to simultaneously determine the content of potential active components in Scutellariae Barbatae Herba and also to provide a reference approach for screening out the differential quality control components among different batches of Scutellariae Barbatae Herba. Chromatographic separations were conducted on a Thermo Acclaim~(TM) RSLC 120 C_(18) column(3.0 mm×100 mm, 2.2 μm) in a gradient program. The mobile phase consisted of 0.1% aqueous formic acid and acetonitrile, and the column temperature was maintained at 40 ℃. The flow rate was 0.4 mL·min~(-1) and the injection volume was 2 μL. The targeted compounds were monitored in the multiple reaction monitoring(MRM) mode. The acquired data were processed by hierarchical cluster analysis(HCA) and partial least square discriminant analysis(PLS-DA). Sixteen compounds all showed good linear relationship within the corresponding linear ranges and the R~2 values were all higher than 0.993 2. The RSDs of precision, repeatability, and stability were less than or equal to 3.7%. Mean recovery rates were in the range of 95.67% and 104.8% with RSDs≤3.2%. According to HCA and PLS-DA, all samples were clustered into four categories. Scutellarin, acteoside, scutellarein, and scutebarbatine X(VIP>1) were considered as differential chemical markers in the four categories. In conclusion, the developed method can be used for the simulta-neous determination of the multiple components and quality control of Scutellariae Barbatae Herba.
