DNA methylation diversity analysis of Lycium barbarum samples from different cultivation areas based on MSAP.
10.19540/j.cnki.cjcmm.20211023.102
- Author:
Fang ZHANG
1
;
You-Yuan LU
2
;
Er-Xin SHANG
1
;
Sheng GUO
1
;
Xue-Jun LU
3
;
Gang REN
4
;
Zhan-Ping CHEN
4
;
Yu-Ling ZHAO
5
;
Da-Wei QIAN
1
;
Jin-Ao DUAN
1
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education, School of Pharmacy, Ningxia Medical University Yinchuan 750021, China.
3. Ningxia Gouqi Innovation Center Yinchuan 750002, China.
4. Haixi Agricultural Science Research Institute of Mongolian and Tibetan Autonomous Prefecture Delingha 817000, China.
5. Jinghe Gouqi Industry Development Center of Bortala Mongolian Autonomous Prefecture Bortala 833399, China.
- Publication Type:Journal Article
- Keywords:
DNA methylation;
Lycium barbarum;
ecological factors;
epigenetics;
methylation-sensitive amplified polymorphisms
- MeSH:
China;
DNA Methylation;
DNA Primers;
Epigenesis, Genetic;
Lycium/genetics*
- From:
China Journal of Chinese Materia Medica
2022;47(2):392-402
- CountryChina
- Language:Chinese
-
Abstract:
Obvious epigenetic differentiation occurred on Lycium barbarum in different cultivation areas in China. To investigate the difference and change rule of DNA methylation level and pattern of L. barbarum from different cultivation areas in China, the present study employed fluorescence-assisted methylation-sensitive amplified polymorphism(MSAP) to analyze the methylation level and polymorphism of 53 genomic DNA samples from Yinchuan Plain in Ningxia, Bayannur city in Inner Mongolia, Jingyuan county and Yumen city in Gansu, Delingha city in Qinghai, and Jinghe county in Xinjiang. The MSAP technical system suitable for the methylation analysis of L. barbarum genomic DNA was established and ten pairs of selective primers were selected. Among amplified 5'-CCGG-3' methylated sites, there were 35.85% full-methylated sites and 39.88% hemi-methylated sites, showing a high degree of epigenetic differentiation. Stoichiometric analysis showed that the ecological environment was the main factor affecting the epigenetic characteristics of L. barbarum, followed by cultivated varieties. Precipitation, air temperature, and soil pH were the main ecological factors affecting DNA methylation in different areas. This study provided a theoretical basis for the analysis of the epigenetic mechanism of L. barbarum to adapt to the diffe-rent ecological environments and research ideas for the introduction, cultivation, and germplasm traceability of L. barbarum.
