Chemical analysis of classical prescription Qianghuo Shengshi Standard Decoction by UHPLC-Q Exactive Orbitrap MS.
10.19540/j.cnki.cjcmm.20210312.301
- Author:
Xin-Fu CAI
1
;
Ya XU
2
;
He-Ping LIU
3
;
Qiang SHANG
3
;
Jia-Qi QIU
2
;
Wen XU
2
Author Information
1. Sichuan Guangda Pharmaceutical Co., Ltd. Pengzhou 611930, China.
2. the Second Clinical College of Guangzhou University of Chinese Medicine Guangzhou 510405, China.
3. Sichuan Guangda Pharmaceutical Co., Ltd. Pengzhou 611930, China National Engineering Research Center for Modernization of Traditional Chinese Medicine Zhuhai 519090, China.
- Publication Type:Journal Article
- Keywords:
Qianghuo Shengshi Standard Decoction;
UHPLC-Q Exactive Orbitrap MS;
chemical constituents;
classical prescription
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Gas Chromatography-Mass Spectrometry;
Mass Spectrometry;
Quality Control
- From:
China Journal of Chinese Materia Medica
2022;47(2):343-357
- CountryChina
- Language:Chinese
-
Abstract:
A UHPLC-Q Exactive Orbitrap MS method was used to analyze the chemical constituents of the classical prescription Qianghuo Shengshi Standard Decoction(QHSS). UHPL conditions were as follows: Waters~(TM) UPLC~(TM) HSS T3 C_(18) column(2.1 mm×100 mm, 1.7 μm) and mobile phase of acetonitrile-0.1% formic acid aqueous solution. Mass spectrometry data of QHSS, each herb extract, and negative sample were collected in both positive and negative ion modes. The chemical constituents of QHSS were identified or tentatively identified based on the accurate molecular weight, retention time, MS fragmentation, comparison with reference substances, and literature reports. A total of 141 compounds were identified, including 18 amino acids, oligosaccharides, oligopeptides, and their derivatives, 19 phenolic acids, 44 coumarins, 18 flavonoids and chromones, 13 saponins, 17 phthalides, and 12 other components. This study comprehensively characterized the chemical constituents of QHSS, laying an experimental basis for the in-depth research on the material basis and quality control of QHSS.
