UPLC fingerprint and determination of five components of substance benchmark of classical prescription Shentong Zhuyu Decoction.
10.19540/j.cnki.cjcmm.20210319.302
- Author:
Lin WANG
1
;
Yan-Ping JIANG
1
;
Hua-Juan JIANG
1
;
Yi CHEN
1
;
Xin NIE
1
;
Xiu-Lan PU
1
;
Chen-Xi ZHAO
2
;
Zhi-Song YANG
2
;
Jin-Ming ZHANG
1
;
Chao-Mei FU
1
Author Information
1. State Key Laboratory of Characteristic Chinese Drug Resources in Southwest China, School of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
2. Anhui Yulong Yadong Pharmaceutical Co., Ltd. Bozhou 236800, China.
- Publication Type:Journal Article
- Keywords:
Shentong Zhuyu Decoction;
UPLC;
classical prescription;
content determination;
fingerprints;
substance benchmark
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/standards*;
Quality Control
- From:
China Journal of Chinese Materia Medica
2022;47(2):334-342
- CountryChina
- Language:Chinese
-
Abstract:
Fingerprints of 18 batches of substance benchmark of Shentong Zhuyu Decoction(SZD) were established by UPLC under the following conditions: Waters Sun Fire C_(18) column(3.0 mm×150 mm, 3.5 μm), column temperature of 35 ℃, gradient elution with mobile phase of acetonitrile(A)-0.1% phosphoric acid aqueous solution(B) at the flow rate of 0.4 mL·min~(-1), and detection by wavelength switching. A total of 16 common peaks were identified. The similarities among the fingerprints were calculated by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition) and the result showed they were in the range of 0.911-0.988. Based on the 16 common peaks, cluster analysis(CA), principal component analysis(PCA), and partial least square discriminant analysis(PLS-DA) all categorized the 18 batches of samples into two groups(S1, S2, S5-S8, S14, and S17 in one group, and S1, S2, S5-S8, S14, and S17 in another), and 11 most influential components were screened. Five known components with great difference among samples(hydroxysafflor yellow A, ferulic acid, benzoic acid, ecdysone, and ammonium glycyrrhizinate) were determined. The combination of multi-component content determination and fingerprints can reflect the overall cha-racteristics of the primary standards of SZD, which is simple, feasible, reproducible, and stable. This study can serve as a reference for the quality control of the primary standards of SZD.
