Quality value transfer of substance benchmarks in Huanglian Decoction.
10.19540/j.cnki.cjcmm.20210412.304
- Author:
Mei-Mei PENG
1
;
Shuang GUO
2
;
Lu WANG
1
;
Jing MAO
1
;
Hui XIE
1
;
Xiao-Li ZHAO
1
;
Jin-Cai HOU
3
;
Yong GUO
3
;
Tu-Lin LU
1
;
Chun-Qin MAO
1
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. Jiangsu Province Hospital of Chinese Medicine Nanjing 210029, China.
3. Shineway Pharmaceutical Group Co., Ltd. Shijiazhuang 051430, China.
- Publication Type:Journal Article
- Keywords:
Huanglian Decoction;
classical prescription;
quality value transfer;
substance benchmarks
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/standards*;
Quality Control
- From:
China Journal of Chinese Materia Medica
2022;47(2):313-323
- CountryChina
- Language:Chinese
-
Abstract:
Following the preparation of substance benchmarks in Huanglian Decoction from 18 batches, the method for detecting their characteristic spectra was established to identify the similarity range and peak attribution. The content and transfer rate ranges of the index components coptisine, palmatine, berberine, liquiritin, glycyrrhizic acid, 6-gingerol, and cinnamaldehyde and the extraction amount were combined for analyzing the quality value transfer from the Chinese medicinal pieces to substance benchmarks and clarifying the key quality attributes of substance benchmarks in Huanglian Decoction. The results showed that the substance benchmarks in Huang-lian Decoction of 18 batches exhibited good similarity in characteristic spectra(all greater than 0.98). There were 17 characteristic peaks identified in the substance benchmarks of Huanglian Decoction, including 10 from Coptidis Rhizoma, 3 from Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle(processed with water), 1 from Zingiberis Rhizoma, and 3 from Cinnamomi Ramulus. The contents and average transfer rates of the index components were listed as follows: coptisine 2.20-6.46 mg·g~(-1) and 18.50%±2.93%; palmatine 3.03-8.13 mg·g~(-1) and 26.56%±4.69%; berberine 7.71-22.29 mg·g~(-1) and 17.34%±3.00%; liquiritin 0.88-2.18 mg·g~(-1) and 9.88%±4.88%; glycyrrhizic acid 1.83-4.44 mg·g~(-1) and 8.50%±3.72%; 6-gingerol 0.56-1.43 mg·g~(-1) and 11.36%±2.37%; cinnamaldehyde 1.55-3.48 mg·g~(-1) and 19.02%±4.36%. The extraction amount of the substance benchmarks from the 18 batches was controlled at 10.65%-13.88%. In this paper, the quality value transfer of substance benchmarks in Huanglian Decoction was analyzed based on the characteristic spectra, the index component contents and the extraction amount, which has provided a basis for the subsequent development of Huanglian Decoction and the quality control of its related preparations.
