Effects of components in stasis-resolving and collateral-dredging Chinese herbal medicines on angiogenesis and inflammatory response of human umbilical vein endothelial cells induced by VEGF.
10.19540/j.cnki.cjcmm.20211012.401
- Author:
Bing LUAN
1
;
Rong YUAN
2
;
Qi-Qi XIN
2
;
Wei-Hong CONG
2
;
Ping SONG
1
Author Information
1. Department of Dermatology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences Beijing 100053, China.
2. Department of Cardiovascular, Xiyuan Hospital, China Academy of Chinese Medical Sciences Beijing 100091, China.
- Publication Type:Journal Article
- Keywords:
angiogenesis;
hydroxy safflower yellow A;
inflammatory response;
paeonol;
psoriasis;
scutellarin
- MeSH:
Angiogenesis Inhibitors/pharmacology*;
China;
Human Umbilical Vein Endothelial Cells;
Humans;
Neovascularization, Pathologic/drug therapy*;
Vascular Endothelial Growth Factor A/metabolism*
- From:
China Journal of Chinese Materia Medica
2022;47(3):737-744
- CountryChina
- Language:Chinese
-
Abstract:
The present study investigated the mechanism of components in stasis-resolving and collateral-dredging Chinese herbal medicines, including scutellarin(Scu), paeonol(Pae), and hydroxy safflower yellow A(HSYA), in the treatment of psoriasis by regulating angiogenesis and inflammation. The human umbilical vein endothelial cells(HUVECs) cultured in vitro were divided into a normal group, a model group, a VEGFR tyrosine kinase inhibitor Ⅱ(VRI) group, and Scu, Pae, and HSYA groups with low, me-dium, and high doses. Cell viability was detected by the CCK-8 assay. Cell migration was detected by wound healing assay. Tube formation assay was used to measure the tube formation ability. Western blot was used to detect the protein expression of the VEGFR2/Akt/ERK1/2 signaling pathway. The secretion levels of inflammatory cytokines IFN-γ, IL-1β, IL-6, and TNF-α were detected by ELISA. The results showed that compared with the model group, all the Scu, Pae, and HSYA groups could reduce cell viability, inhibit cell migration and tube formation(P<0.05, P<0.01), and down-regulated the protein expression of VEGFR2, p-VEGFR2, Akt, p-Akt, ERK1/2, and p-ERK1/2. Scu and Pae could down-regulate VEGFR2 expression(P<0.05, P<0.01), while other groups only showed a downward trend. Scu and Pae significantly reduced IFN-γ and IL-6 levels(P<0.01), and HSYA significantly reduced the levels of IFN-γ, IL-1β, and IL-6(P<0.01). Scu, Pae, and HSYA had no significant effect on TNF-α. The results suggested that Scu, Pae, and HSYA may exert a therapeutic role in psoriasis-related angiogenesis and inflammation by inhibiting VEGFR2/Akt/ERK1/2 signaling pathway and inhibiting the secretion of IFN-γ, IL-1β, and IL-6.
