~1H-qNMR quantification of total ginsenosides in Shenmai Injection.
10.19540/j.cnki.cjcmm.20211108.302
- Author:
Wen-Zhu LI
1
;
Jia-Yu YANG
1
;
Fang ZHAO
2
;
Jian-Yang PAN
1
;
Hai-Bin QU
1
Author Information
1. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University Hangzhou 310058, China Innovation Center in Zhejiang University, State Key Laboratory of Component-Based Chinese Medicine Hangzhou 310058,China.
2. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University Hangzhou 310058, China Innovation Center in Zhejiang University, State Key Laboratory of Component-Based Chinese Medicine Hangzhou 310058,China Hisun Biopharmaceutical Co., Ltd. Hangzhou 311400, China.
- Publication Type:Journal Article
- Keywords:
Shenmai Injection;
nuclear magnetic resonance;
quantification method;
total ginsenosides;
~1H-qNMR
- MeSH:
Drug Combinations;
Drugs, Chinese Herbal;
Ginsenosides/analysis*;
Quality Control
- From:
China Journal of Chinese Materia Medica
2022;47(3):587-592
- CountryChina
- Language:Chinese
-
Abstract:
A content determination method based on ~1H-qNMR was developed for the determination of total ginsenosides in Shenmai Injection. The parameters were optimized with CD_3OD as the solvent, dimethyl terephthalate as the internal standard, the peak at δ 8.11 as the internal standard peak, and the peaks at δ 1.68 and δ 0.79 as quantitative peaks of total ginsenosides. The developed ~1H-qNMR-based method was validated methodologically. The results showed that the method could achieve accurate measurement of total ginsenosides in Shenmai Injection in the range of 0.167 6-3.091 1 mmol·L~(-1). The developed ~1H-qNMR-based method for total ginsenosides is simple in operation, short in analysis time, strong in specificity, independent of accompanying standard curve, and small in sample volume, which can serve as a reliable mean for the quality control of Shenmai Injection. This study is expected to provide new ideas for the development of quantification methods of total ginsenosides.
