Quality evaluation of ginsenoside reference substances based on qNMR spectroscopy.
10.19540/j.cnki.cjcmm.20211108.304
- Author:
Jian-Yang PAN
1
;
Fang ZHAO
2
;
Wen-Zhu LI
1
;
Hai-Bin QU
1
Author Information
1. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences,Zhejiang University Hangzhou 310058, China Innovation Center in Zhejiang University, State Key Laboratory of Component-Based Chinese Medicine Hangzhou 310058, China.
2. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences,Zhejiang University Hangzhou 310058, China Innovation Center in Zhejiang University, State Key Laboratory of Component-Based Chinese Medicine Hangzhou 310058, China Hisun Biopharmaceutical Co., Ltd. Hangzhou 311400, China.
- Publication Type:Journal Article
- Keywords:
dimethyl terephthalate;
ginsenoside Rb_1;
ginsenoside Rd;
ginsenoside Re;
ginsenoside Rg_1;
notoginsenoside R_1;
qNMR
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Ginsenosides/analysis*;
Magnetic Resonance Spectroscopy/methods*;
Proton Magnetic Resonance Spectroscopy;
Reference Standards
- From:
China Journal of Chinese Materia Medica
2022;47(3):575-580
- CountryChina
- Language:Chinese
-
Abstract:
The present study established a quality evaluation method for ginsenoside reference substances based on quantitative nuclear magnetic resonance(qNMR) spectroscopy. ~1H-NMR spectra were collected on Bruker Avance Ⅲ 500 MHz NMR spectrometer equipped with a 5 mm BBO probe. The acquire parameters were set up as follows: pulse sequence of 30°, D_1=20 s, probe temperature= 303 K, and the scan number = 32. Dimethyl terephthalate, a high-quality ~1H-qNMR standard, was used as the internal standard and measured by the absolute quantitative method. Methyl peaks of comparatively good sensitivity were selected for quantification, and linear fitting deconvolution was adopted to improve the accuracy of integration results. The qNMR spectroscopy-based method was established and validated, which was then used for the quality evaluation of ginsenoside Rg_1, ginsenoside Re, ginsenoside Rb_1, ginsenoside Rd, and notoginsenoside R_1. The results suggested that the content of these ginsenoside reference standards obtained from the qNMR spectroscopy-based method was lower than that detected by the normalization method in HPLC provided by the manufacturers. In conclusion, the qNMR spectroscopy-based method can ensure the quality of ginsenoside reference substances and provide powerful support for the accurate quality evaluation of Chinese medicine and its preparations. The qNMR spectroscopy-based method is simple, rapid, and accurate, which can be developed for the quantitative assay of Chinese medicine standard references.
