Determination of eight active components of Bufei Huoxue Capsules in rat plasma and their pharmacokinetics by UHPLC-MS/MS.
10.19540/j.cnki.cjcmm.20211109.201
- Author:
Hui REN
1
;
Sheng GUO
1
;
Yi-Ying ZHANG
1
;
Quan LI
2
;
Heng-Bin WANG
2
;
Wan-Li GENG
3
;
Er-Xin SHANG
1
;
Da-Wei QIAN
1
;
Jin-Ao DUAN
1
Author Information
1. National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. Lei Yun Shang Pharmaceutical Group Co., Ltd. Suzhou 215003, China.
3. Guangdong Leiyunshang Pharmaceutical Co., Ltd. Yunfu 527300, China.
- Publication Type:Journal Article
- Keywords:
Astragali Radix;
Bufei Huoxue Capsules;
Psoraleae Fructus;
UHPLC-MS/MS;
pharmacokinetics
- MeSH:
Animals;
Capsules;
Chromatography, High Pressure Liquid/methods*;
Drugs, Chinese Herbal/pharmacokinetics*;
Rats;
Rats, Sprague-Dawley;
Reproducibility of Results;
Tandem Mass Spectrometry/methods*
- From:
China Journal of Chinese Materia Medica
2022;47(1):215-223
- CountryChina
- Language:Chinese
-
Abstract:
An ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) method was established to investigate the pharmacokinetic behaviors of psoralenoside, isopsoralenoside, calycosin-7-glucoside, ononin, psoralen, isopsoralen, methylnissolin, and neobavaisoflavone in rat plasma after oral administration of Bufei Huoxue Capsules. After SD rats were administered with Bufei Huoxue Capsules suspension by gavage, blood samples were collected from the inner canthus at different time points. After protein precipitation, plasma samples were separated on ACQUITY UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm). The mobile phase consisted of acetonitrile(A) and water(B) containing 0.1% formic acid in gradient elution. The positive and negative ions were measured simultaneously in the multi-reaction monitoring(MRM) mode. The pharmacokinetic parameters were calculated and fitted by DAS 3.2.8. Psoralenoside, isopsoralenoside, calycosin-7-glucoside, ononin, psoralen, isopsoralen, methylnissolin, and neobavaisoflavone were detected in the rat plasma after drug administration, with AUC_(0-t) of(3 357±1 348),(3 555±1 696),(3.03±0.88),(2.21±0.33),(1 787±522),(2 295±539),(5.69±1.41) and(3.40±0.75) μg·L~(-1)·h, and T_(max) of(1.56±0.62),(1.40±0.70),(0.21±0.05),(0.25±0.12),(0.26±0.11),(0.34±0.29),(0.74±0.59), and 0.25 h. The method is proved specific and repeatable and is suitable for the determination of psoralenoside, isopsoralenoside, calycosin-7-glucoside, ononin, pso-ralen, isopsoralen, methylnissolin, and neobavaisoflavone in the rat plasma, which can be applied to pharmacokinetic study.
